Rapid and Sensitive Determination of Phenol in Honey by High-Performance Liquid Chromatography with Fluorescence Detection

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• 作者：Má ; rta Gy&oacute ; rik ; Zoltá ; n Herpai ; Ildik&oacute ; Szé ; csé ; nyi ; Lá ; szl&oacute ; Varga ; and Jen&oacute ; Szigeti
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2003
• 出版时间：August 27, 2003
• 年：2003
• 卷：51
• 期：18
• 页码：5222 - 5225
• 全文大小：79K
• 年卷期：v.51,no.18(August 27, 2003)
• ISSN：1520-5118

文摘

The objective of this research was to develop a novel high-performance liquid chromatographic (HPLC)method involving a simple sample preparation procedure for the rapid, low-cost, and sensitivequantitation of phenol in honey at levels of regulatory and practical importance. After proper dilutionof honey with water, the samples were analyzed by a gradient HPLC system, using a reversed-phase column with fluorescence detection at excitation and emission wavelengths of 270 and 300nm, respectively. The eluents applied were water-acetonitrile-85% orthophosphoric acid (10:10:0.01, v/v/v) and water-85% orthophosphoric acid (20:0.01, v/v). The retention time of phenol wasfound to be 14.1 min, and the limit of quantitation for phenol in honey was set at 5 g/kg. Overallrecovery was 98%. The proposed method has been successfully applied to real sample analysis.Keywords: Phenol; honey; sample preparation; HPLC; fluorescence detection