Evaluation of Fumonisin Contamination in Cornflakes on the Belgian Market by "Flow-Through" Assay Screening and LC-MS/MS Analyses

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• 作者：Charline Paepens ; Sarah De Saeger ; Liberty Sibanda ; Ildik&oacute ; Barna-Vetr&oacute ; Marianne Anselme ; Yvan Larondelle ; and Carlos Van Peteghem
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：September 7, 2005
• 年：2005
• 卷：53
• 期：18
• 页码：7337 - 7343
• 全文大小：230K
• 年卷期：v.53,no.18(September 7, 2005)
• ISSN：1520-5118

文摘

A total of 205 cornflake samples collected in Belgian retail stores during 2003-2004 were surveyedfor the natural occurrence of fumonisin B₁ (FB₁), B₂ (FB₂), and B₃ (FB₃). These cornflake samples,originating from conventional as well as from organic production, were analyzed using an intralaboratory-validated LC-MS/MS method. Additionally, 90 cornflake samples were subjected to rapidscreening using a flow-through enzyme immunoassay method to demonstrate the practicability of ascreening test coupled to a validated confirmatory LC-MS/MS method for the management of foodsafety risks. FB₁ concentrations ranged from not detected (nd) [LOD (FB₁) = 20 g/kg] to 464 g/kgwith mean and median concentrations of respectively 104 ± 113 and 54 g/kg. For FB₂ and FB₃, theconcentration ranges varied respectively from nd [LOD (FB₂) = 7.5 g/kg] to 43 g/kg and from nd[LOD (FB₃) = 12.5 g/kg] to 90 g/kg. Mean concentrations for FB₂ and FB₃ were respectively 12 ±8 and 21 ± 15 g/kg, while the median concentration was 11 g/kg for FB₂ and 19 g/kg for FB₃.From the statistical tests (² and ANOVA model III), it could be concluded that the agricultural practicedid not have any significant effect on the fumonisin concentrations but that the variation betweendifferent batches was significant (p < 0.0001).Keywords: Fumonisins, cornflakes, rapid field test, LC-MS/MS, conventional/organic food