Thermodynamic Parameters Based on a Nearest-Neighbor Model for DNA Sequences with a Single-Bulge Loop
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- 作者：Fumiaki Tanaka ; Atsushi Kameda ; Masahito Yamamoto ; and Azuma Ohuchi
- 刊名：Biochemistry
- 出版年：2004
- 出版时间：June 8, 2004
- 年：2004
- 卷：43
- 期：22
- 页码：7143 - 7150
- 全文大小：68K
- 年卷期：v.43,no.22(June 8, 2004)
- ISSN：1520-4995
文摘
All 64 possible thermodynamic parameters for a single-bulge loop in the middle of a sequencewere derived from optical melting studies. The relative stability of a single bulge depended on both thetype of bulged base and its flanking base pairs. The contribution of the single bulge to helix stabilityranged from 3.69 kcal/mol for a TAT bulge to -1.05 kcal/mol for an ACC bulge. Thermodynamics for10 sequences with a GTG bulge were determined to test the applicability of the nearest-neighbor modelto a single-bulge loop. Thermodynamic parameters for the GTG bulge and Watson-Crick base pairspredict 
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