Use of Limonene in Countercurrent Chromatography: A Green Alkane Substitute

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• 作者：Karine Faure ; Elodie Bouju ; Pauline Suchet ; Alain Berthod
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：May 7, 2013
• 年：2013
• 卷：85
• 期：9
• 页码：4644-4650
• 全文大小：336K
• 年卷期：v.85,no.9(May 7, 2013)
• ISSN：1520-6882

文摘

Counter-current chromatography (CCC) is a preparative separation technique working with the two liquid phases of a biphasic liquid system. One phase is used as the mobile phase when the other, the stationary phase, is held in place by centrifugal fields. Limonene is a biorenewable cycloterpene solvent coming from orange peel waste. It was evaluated as a possible substitute for heptane in CCC separations. The limonene/methanol/water and heptane/methanol/water phase diagrams are very similar at room temperature. The double bonds of the limonene molecule allows for possible 蟺鈥撓€ interactions with solutes rendering limonene slightly more polar than heptane giving small differences in solute partition coefficients in the two systems. The 24% higher limonene density is a difference with heptane that has major consequences in CCC. The polar and apolar phases of the limonene/methanol/water 10/9/1 v/v have 鈭?.025 g/cm³ density difference (lower limonene phase) compared to +0.132 g/cm³ with heptane (upper heptane phase). This precludes the use of this limonene system with hydrodynamic CCC columns that need significant density difference to retain a liquid stationary phase. It is an advantage with hydrostatic CCC columns because density difference is related to the working pressure drop: limonene allows one to work with high centrifugal fields and moderate pressure drop. Limonene has the capability to be a 鈥済reen鈥?alternative to petroleum-based solvents in CCC applications.