Controlled Manipulation of Bacteriophages Using Single-Virus Force Spectroscopy
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- 作者：David Alsteens ; Emanuele Pesavento ; Gilles Cheuvart ; Vincent Dupres ; Heykel Trabelsi ; Patrice Soumillion ; Yves F. Dufrêne
- 刊名：ACS Nano
- 出版年：2009
- 出版时间：October 27, 2009
- 年：2009
- 卷：3
- 期：10
- 页码：3063-3068
- 年卷期：0
- ISSN：1936-086X
文摘
A method is described for the site-directed manipulation of single filamentous bacteriophages, by using phage display technology and atomic force microscopy. f1 filamentous bacteriophages were genetically engineered to display His-tags on their pIX tail. Following adsorption on nitrilotriacetate-terminated surfaces, force spectroscopy with tips bearing monoclonal anti-pIII antibodies was used to pull on individual phages via their pIII head. Analysis of the force-extension profiles revealed that upon pulling, the phages are progressively straightened into an extended orientation until rupture of the anti-pIII/pIII complex. The single-virus manipulation technique presented here provides new opportunities for understanding the forces driving cell−virus and material−virus interactions, and for characterizing the binding properties of polypeptide sequences or proteins selected by the phage display technology.