Electrophoretic Interpretation of PEGylated NP Structure with and without Peripheral Charge

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• 作者：Reghan J. Hill ; Fei Li ; Tennyson L. Doane ; Clemens Burda
• 刊名：Langmuir
• 出版年：2015
• 出版时间：September 22, 2015
• 年：2015
• 卷：31
• 期：37
• 页码：10246-10253
• 全文大小：359K
• ISSN：1520-5827

文摘

Anchoring poly(ethylene glycol) (PEG) to inorganic nanoparticles (NPs) permits control over NP properties for a variety of technological applications. However, the core鈥搒hell structure tremendously complicates the interpretation of the ubiquitous 味-potential, as furnished by electrophoretic light-scattering, capillary electrophoresis or gel electrophoresis. To advance the 味-potential鈥攁nd the more fundamental electrophoretic mobility鈥攁s a quantitative diagnostic for PEGylated NPs, we synthesized and characterized Au NPs bearing terminally anchored 5 kDa PEG ligands with univalent carboxymethyl end groups. Using the electrophoretic mobilities, acquired over a wide range of ionic strengths, we developed a theoretical model for the distributions of polymer segments, charge, electrostatic potential, and osmotic pressure that envelop the core: knowledge that will help to improve the performance of soft NPs in fundamental research and technological applications.