Effect of Surface Parameters on the Performance of IgG-Arrayed Hydrogel Chips: A Comprehensive Study

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• 作者：Katarzyna Derwinska ; Levi A. Gheber ; Ursula Sauer ; Leopold Schorn ; Claudia Preininger
• 刊名：Langmuir
• 出版年：2007
• 出版时间：October 9, 2007
• 年：2007
• 卷：23
• 期：21
• 页码：10551 - 10558
• 全文大小：344K
• 年卷期：v.23,no.21(October 9, 2007)
• ISSN：1520-5827

文摘

In this article, the assay performance of 3D polyurethane (PU) hydrogel surfaces, used either plain or modifiedwith cross linkers and additives in a direct immunoassay for IgG, is correlated with chip surface parameters such aswater content and expansion, mechanical stability, hydrophilicity, thickness, and surface topography. Commercial chipsurfaces ARChip Epoxy, Nexterion slide H and HydroGel are used as references. A strong correlation between assaysensitivity and physical surface parameters was found only for various hydrogels of the same chemical composition,in which cases assay sensitivity increases with decreasing hydrogel concentration as well as decreasing roughness,water content, and expansion. However, as is the case with all hydrogels tested, more hydrophobic layers with lowwater content are more highly reproducible from one measurement to another.