Characterization and Activity of Anthocyanins in Zijuan Tea (Camellia sinensis var. kitamura)

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• 作者：Lihua Jiang ; Xiaojia Shen ; Toshihiko Shoji ; Tomomasa Kanda ; Jiachun Zhou ; Liming Zhao
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：April 3, 2013
• 年：2013
• 卷：61
• 期：13
• 页码：3306-3310
• 全文大小：225K
• 年卷期：v.61,no.13(April 3, 2013)
• ISSN：1520-5118

文摘

Zijuan tea is a new cultivar produced in Yunnan province of China. Unlike most tea cultivars, Zijuan tea is anthocyanin-rich. The composition and antioxidant activities of anthocyanins of Zijuan tea were studied for the first time in this paper. Anthocyanins were extracted with acidified methanol and quantified as 707 卤 28 渭g/g of dry weight (cyanidin-3-O-尾-d-glucoside equivalent) by high-performance liquid chromatography (HPLC) analysis. Four anthocyanins were successfully identified after Amberlite XAD-7HP adsorption column chromatography and octadecyl silane (ODS) flash chromatography. Among the four, delphinidin-3-O-尾-d-galactoside (1) and cyanidin-3-O-尾-d-galactoside (2) were confirmed by liquid chromatography鈥揺lectrospray ionization mass spectrometry (LC-ESI-MS) and HPLC. Delphinidin-3-O-尾-d-(6-(E)-p-coumaroyl) galactopyranoside (3) and cyanidin-3-O-尾-d-(6-(E)-p-coumaroyl) galactopyranoside (4) were characterized by the high-resolution time-of-flight-mass spectrometry (HRTOF-MS) and nuclear magnetic resonance (NMR) spectrometry. The antioxidant activities of compounds 3 and 4, which composed approximately 75% of the total anthocyanins content in HPLC analysis, were evaluated by DPPH and FRAP assays. Results showed that both had higher antioxidant activities than commercial antioxidants butylated hydroxytoluene (BHT) used as one of controls in these assays.

Keywords:

Zijuan tea; anthocyanin; antioxidant activity; LC-ESI-MS; NMR