Hierarchical Assembly of Model Cell Surfaces: Synthesis of Mucin Mimetic Polymers and Their Display on Supported Bilayers

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• 作者：David Rabuka ; Raghuveer Parthasarathy ; Goo Soo Lee ; Xing Chen ; Jay T. Groves ; Carolyn R. Bertozzi
• 刊名：Journal of the American Chemical Society
• 出版年：2007
• 出版时间：May 2, 2007
• 年：2007
• 卷：129
• 期：17
• 页码：5462 - 5471
• 全文大小：487K
• 年卷期：v.129,no.17(May 2, 2007)
• ISSN：1520-5126

文摘

Molecular level analysis of cell-surface phenomena could benefit from model systems comprisingstructurally defined components. Here we present the first step toward bottom-up assembly of model cellsurfaces-the synthesis of mucin mimetics and their incorporation into artificial membranes. Natural mucinsare densely glycosylated O-linked glycoproteins that serve numerous functions on cell surfaces. Their largesize and extensive glycosylation makes the synthesis of these biopolymers impractical. We designedsynthetically tractable glycosylated polymers that possess rodlike extended conformations similar to naturalmucins. The glycosylated polymers were end-functionalized with lipid groups and embedded into supportedlipid bilayers where they interact with protein receptors in a structure-dependent manner. Furthermore,their dynamic behavior in synthetic membranes mirrored that of natural biomolecules. This system providesa unique framework with which to study the behavior of mucin-like macromolecules in a controlled, cellsurface-mimetic environment.