Impact of Fullerene (C60) on a Soil Microbial Community
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- 作者：Zhonghua Tong ; Marianne Bischoff ; Loring Nies ; Bruce Applegate ; Ronald F. Turco
- 刊名：Environmental Science & Technology
- 出版年：2007
- 出版时间：April 15, 2007
- 年：2007
- 卷：41
- 期：8
- 页码：2985 - 2991
- 全文大小：312K
- 年卷期：v.41,no.8(April 15, 2007)
- ISSN：1520-5851
文摘
The nascent state of the nanoproduct industry calls forimportant early assessment of environmental impacts beforesignificant releases have occurred. Clearly, the impactof manufactured nanomaterials on key soil processes mustbe addressed so that an unbiased discussion concerningthe environmental consequences of nanotechnologycan take place. In this study, soils were treated with either1 
g C60 g-1 soil in aqueous suspension (nC60) or 1000g C60 g-1 soil in granular form, a control containing equivalenttetrahydrofuran residues as generated during nC60formation process or water and incubated for up to 180days. Treatment effects on soil respiration, both basal andglucose-induced, were evaluated. The effects on the soilmicrobial community size was evaluated using totalphospholipid derived phosphate. The impact on communitystructure was evaluated using both fatty acid profilesand following extraction of total genomic DNA, by DGGEafter PCR amplification of total genomic DNA using bacterialvariable V3 region targeted primers. In addition, treatmentaffects on soil enzymatic activities for 
-glucosidase, acid-phosphatase, dehydrogenase, and urease were followed.Our observations show that the introduction of fullerene, aseither C60 or nC60, has little impact on the structure andfunction of the soil microbial community and microbialprocesses.
g C60 g-1 soil in aqueous suspension (nC60) or 1000g C60 g-1 soil in granular form, a control containing equivalenttetrahydrofuran residues as generated during nC60formation process or water and incubated for up to 180days. Treatment effects on soil respiration, both basal andglucose-induced, were evaluated. The effects on the soilmicrobial community size was evaluated using totalphospholipid derived phosphate. The impact on communitystructure was evaluated using both fatty acid profilesand following extraction of total genomic DNA, by DGGEafter PCR amplification of total genomic DNA using bacterialvariable V3 region targeted primers. In addition, treatmentaffects on soil enzymatic activities for -glucosidase, acid-phosphatase, dehydrogenase, and urease were followed.Our observations show that the introduction of fullerene, aseither C60 or nC60, has little impact on the structure andfunction of the soil microbial community and microbialprocesses.