Site-Specific Attachment of a Protein to a Carbon Nanotube End without Loss of Protein Function
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- 作者：Shige H. Yoshimura ; Shahbaz Khan ; Satoshi Ohno ; Takashi Yokogawa ; Kazuya Nishikawa ; Takamitsu Hosoya ; Hiroyuki Maruyama ; Yoshikazu Nakayama ; Kunio Takeyasu
- 刊名：Bioconjugate Chemistry
- 出版年：2012
- 出版时间：July 18, 2012
- 年：2012
- 卷：23
- 期：7
- 页码：1488-1493
- 全文大小：298K
- 年卷期：v.23,no.7(July 18, 2012)
- ISSN：1520-4812
文摘
Establishing a nanobiohybrid device largely relies on the availability of various bioconjugation procedures which allow coupling of biomolecules and inorganic materials. Especially, site-specific coupling of a protein to nanomaterials is highly useful and significant, since it can avoid adversely affecting the protein鈥檚 function. In this study, we demonstrated a covalent coupling of a protein of interest to the end of carbon nanotubes without affecting protein鈥檚 function. A modified Staudinger-Bertozzi ligation was utilized to couple a carbon nanotube end with an azide group which is site-specifically incorporated into a protein of interest. We demonstrated that Ca2+-sensor protein, calmodulin, can be attached to the end of the nanotubes without affecting the ability to bind to the substrate in a calcium-dependent manner. This procedure can be applied not only to nanotubes, but also to other nanomaterials, and therefore provides a fundamental technique for well-controlled protein conjugation.