Determination of Geochemically Important Sterols and Triterpenols in Sediments Using Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry (UHPLC鈥揗S/MS)
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- 作者：Giovana Anceski Bataglion ; Eduardo Meurer ; Ana Cec铆lia Rizzatti de Albergaria-Barbosa ; M谩rcia Caruso B铆cego ; Rolf Roland Weber ; Marcos Nogueira Eberlin
- 刊名：Analytical Chemistry
- 出版年：2015
- 出版时间：August 4, 2015
- 年：2015
- 卷：87
- 期：15
- 页码：7771-7778
- 全文大小：443K
- ISSN：1520-6882
文摘
A fast, sensitive, and selective ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC鈥揗S/MS) method that is able to quantify geochemical biomarkers in sediment is described. A pool of 10 sterols, which can be used as biomarkers of autochthonous (cholesterol, cholestanol, brassicasterol, ergosterol), allochthonous (stigmasterol, 尾-sitosterol, campesterol, and stigmastanol) and anthropogenic (coprostanol and epicoprostanol) organic matter (OM), and three triterpenols (lupeol, 伪-amyrin, and 尾-amyrin) were chosen as the analytes. The method showed excellent analytical parameters, and, compared with the traditional GC鈥揗S methods that are commonly applied for the analysis of sterols, this method requires no sample cleanup or derivatization and presents improved values for the LOD and LOQ. UHPLC can separate the diastereoisomers (epicoprostanol, coprostanol, and cholestanol) and the isomers (lupeol, 伪-amyrin, and 尾-amyrin). The method was successfully applied for the quantification of the biomarkers, and thus, it was applied to assess the OM sources and the impacts of anthropogenic activities in sediments from different environments, such as Antarctica and other Brazilian systems (Continental Shelf, S茫o Sebasti茫o Channel, and Santos Estuary). Unique profiles of the biomarkers were observed for the contrasting environments, and 尾-amyrin and cholesterol were more predominant in the Santos Estuary and Antarctica samples, respectively. The sterol ratios indicated a higher level of sewage contamination in the Santos Estuary.