Biochemical Evolution of DNA Polymerase η: Properties of Plant, Human, and Yeast Proteins
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- 作者：Peter D. Hoffman ; Marc J. Curtis ; Shigenori Iwai ; John B. Hays
- 刊名：Biochemistry
- 出版年：2008
- 出版时间：April 22, 2008
- 年：2008
- 卷：47
- 期：16
- 页码：4583 - 4596
- 全文大小：5658K
- 年卷期：v.47,no.16(April 22, 2008)
- ISSN：1520-4995
文摘
To assess how evolution might have biochemically shaped DNA polymerase η (Polη) in plants, we expressed in Escherichia coli proteins from Arabidopsis thaliana (At), humans (Hs), and the yeast Saccharomyces cerevisiae (Sc), purified them to near homogeneity, and compared their properties. Consistent with the multiple divergent amino acids within mostly conserved polymerase domains, the polymerases showed modest, appreciable, and marked differences, respectively, in salt and temperature optima for activity and thermostability. We compared abilities to extend synthetic primers past template cyclobutane thymine dimers (T[CPD]T) or undamaged T-T under physiological conditions (80–110 mM salt). Specific activities for “standing-start” extension of synthetic primers ending opposite the second template nucleotide 3′ to T-T were roughly similar. During subsequent “running-start” insertions past T-T and the next 5′ (N + 1) nucleotide, AtPolη and HsPolη appeared more processive, but DNA sequence contexts strongly affected termination probabilities. Lesion-bypass studies employed four different templates containing T[CPD]Ts, and two containing pyrimidine (6-4′)-pyrimidinone photoproducts ([6-4]s). AtPolη made the three successive insertions [opposite the T[CPD]T and (N + 1) nucleotides] that define bypass nearly as well as HsPolη and somewhat better than ScPolη. Again, sequence context effects were profound. Interestingly, the level of insertion opposite the (N − 1) nucleotide 3′ to T[CPD]T by HsPolη and especially AtPolη, but not ScPolη, was reduced (up to 4-fold) relative to the level of insertion opposite the (N − 1) nucleotide 3′ to T-T. Evolutionary conservation of efficient T[CPD]T bypass by HsPolη and AtPolη may reflect a high degree of exposure of human skin and plants to solar UV-B radiation. The depressed (N − 1) insertion upstream of T[CPD]T (but not T-T) may reduce the extent of gratuitous error-prone insertion.