Proteomic Profile of Edible Bird鈥檚 Nest Proteins

详细信息    查看全文

• 作者：Xiaoqing Liu ; Xintian Lai ; Shiwei Zhang ; Xiuli Huang ; Quanxue Lan ; Yun Li ; Bifang Li ; Wei Chen ; Qinlei Zhang ; Dezhi Hong ; Guowu Yang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：December 26, 2012
• 年：2012
• 卷：60
• 期：51
• 页码：12477-12481
• 全文大小：324K
• 年卷期：v.60,no.51(December 26, 2012)
• ISSN：1520-5118

文摘

Edible bird鈥檚 nest (EBN) is made of the swiftlets鈥?saliva, which has attracted rather more attention owing to its nutritious and medical properties. Although protein constitutes the main composition and plays an important role in EBN, few studies have focused on the proteomic profile of EBN. The purpose of this study was to produce a proteomic map and clarify common EBN proteins. Liquid-phase isoelectric focusing (LIEF) was combined with two-dimensional electrophoresis (2-DE) for comprehensive analysis of EBN proteins. From 20 to 100 protein spots were detected on 2-DE maps of EBN samples from 15 different sources. The proteins were mainly distributed in four taxa (A, B, C, and D) according to their molecular mass. Taxa A and D both contained common proteins and proteins that may be considered another characteristic of EBN. Taxon A was identified using MALDI-TOF-TOF/MS and found to be homologous to acidic mammalian chitinase-like (Meleagris gallopavo), which is in glycosyl hydrolase family 18.

Keywords:

edible bird鈥檚 nest; protein; 2-DE; acidic mammalian chitinase; liquid-phase isoelectric focusing