Fe- and Ln-DOTAm-F12 Are Effective Paramagnetic Fluorine Contrast Agents for MRI in Water and Blood

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• 作者：Kriti Srivastava ; Evan A. Weitz ; Katie L. Peterson ; Małgorzata Marjańska ; Valérie C. Pierre
• 刊名：Inorganic Chemistry
• 出版年：2017
• 出版时间：February 6, 2017
• 年：2017
• 卷：56
• 期：3
• 页码：1546-1557
• 全文大小：585K
• ISSN：1520-510X

文摘

A series of fluorinated macrocyclic complexes, M-DOTAm-F12, where M is Lap>IIIp>, Eup>IIIp>, Gdp>IIIp>, Tbp>IIIp>, Dyp>IIIp>, Hop>IIIp>, Erp>IIIp>, Tmp>IIIp>, Ybp>IIIp>, and Fep>IIp>, was synthesized, and their potential as fluorine magnetic resonance imaging (MRI) contrast agents was evaluated. The high water solubility of these complexes and the presence of a single fluorine NMR signal, two necessary parameters for in vivo MRI, are substantial advantages over currently used organic polyfluorocarbons and other reported paramagnetic p>19p>F probes. Importantly, the sensitivity of the paramagnetic probes on a per fluorine basis is at least 1 order of magnitude higher than that of diamagnetic organic probes. This increased sensitivity is due to a substantial—up to 100-fold—decrease in the longitudinal relaxation time (T₁) of the fluorine nuclei. The shorter T₁ allows for a greater number of scans to be obtained in an equivalent time frame. The sensitivity of the fluorine probes is proportional to the T₂/T₁ ratio. In water, the optimal metal complexes for imaging applications are those containing Hop>IIIp> and Fep>IIp>, and to a lesser extent Tmp>IIIp> and Ybp>IIIp>. Whereas T₁ of the lanthanide complexes are little affected by blood, the T₂ are notably shorter in blood than in water. The sensitivity of Ln-DOTAm-F12 complexes is lower in blood than in water, such that the most sensitive complex in water, Hop>IIIp>-DOTAm-F12, could not be detected in blood. Tmp>IIIp> yielded the most sensitive lanthanide fluorine probe in blood. Notably, the relaxation times of the fluorine nuclei of Fep>IIp>-DOTAm-F12 are similar in water and in blood. That complex has the highest T₂/T₁ ratio (0.57) and the lowest limit of detection (300 μM) in blood. The combination of high water solubility, single fluorine signal, and high T₂/T₁ of M-DOTAm-F12 facilitates the acquisition of three-dimensional magnetic resonance images.