Liver fibrosis is a chronic liver disease that will further develop to cirrhosis if severe damage continuesto form. A potential treatment for liver fibrosis is to inhibit activated hepatic
stellate cell (HSC)proliferation and, subsequently, to induce HSC apoptosis. It has been reported that antioxidants areable to inhibit the proliferation of HSCs. In this study, the aqueous extract of spirulina was chosen asthe source of antioxidant to investigate the inhibitory effect on the proliferation of HSC. The growthinhibitory effects of aqueous spirulina and chlorella extract on human liver cancer cells, HepG2, werealso studied and compared in pairs. Results indicated that the total phenol content of spirulina wasalmost five times greater than that of chlorella (6.86 ± 0.58 vs 1.44 ± 0.04 mg tannic acid equivalent/gof algae powder, respectively). The antioxidant activity of spirulina determined by the ABTS
+ methodwas higher than chlorella (EC
50: 72.44 ± 0.24
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mol of trolox equivalent/g of spirulina extract vs56.09 ± 1.99
![](/images/entities/mgr.gif)
mol of trolox equivalent/g of chlorella extract). Results of DPPH
![](/images/entities/bull.gif)
assay also showeda similar trend as the ABTS
+ assay (EC
50: 19.39 ± 0.65
![](/images/entities/mgr.gif)
mol of ascorbic acid equivalent/g of spirulinaextract vs 14.04 ± 1.06
![](/images/entities/mgr.gif)
mol of ascorbic acid equivalent/g of chlorella extract). The aqueous extractsof these two algae both showed antiproliferative effects on HSC and HepG2, but spirulina was astronger inhibitor than chlorella. Annexin-V staining showed that aqueous extract of spirulina inducedapoptosis of HSC after 12 h of treatment. In addition, the aqueous extract of spirulina triggered a cellcycle arrest of HSC at the G2/M phase.Keywords: Spirulina; chlorella; antioxidant; activated hepatic
stellate cells; liver fibrosis; apoptosis;phenolics