Rapid Purification of Fumonisins and Their Hydrolysis Products with Solid-Phase Extraction Columns
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- 作者：Stephen M. Poling and Ronald D. Plattner
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：1999
- 出版时间：June 1999
- 年：1999
- 卷：47
- 期：6
- 页码：2344 - 2349
- 全文大小：87K
- 年卷期：v.47,no.6(June 1999)
- ISSN：1520-5118
文摘
Fumonisins B3 and B4 (FB3 and FB4) were recovered from the 50:50 acetonitrile/water extract ofcorn cultures of a strain of Fusarium moniliforme that does not make FB1 or FB2 by stirring theextract with IRA-68, a weak anion-exchange resin. The fumonisins were desorbed with 5% aceticacid in the same solvent. After dilution with water, the desorbed fumonisins were separated intoFB3 (FB3 and FA3) and FB4 (FB4, FC4, and FA4) fractions with a tC18 solid-phase extraction (SPE)cartridge. The FB3 fraction was then separated into FB3 and FA3 by using an NH2 SPE cartridgeand eluting with 5% acetic acid and increasing amounts of acetonitrile in water. Finally, FB1 andFA3 were hydrolyzed with calcium hydroxide. After recovery from the reaction mixture using a tC18cartridge, the hydrolyzed and partially hydrolyzed analogues were separated and the unreactedfumonisins recovered by using an NH2 cartridge, initially in the normal-phase mode with increasingamounts of water in acetonitrile and then in the reversed-phase mode after the addition of 5% aceticacid to the solvent and eluting in the reverse order.Keywords: Fumonisins; Fusarium moniliforme; corn; hydrolysis; isolation