Monitoring the Mode of Action of Antibiotics Using Raman Spectroscopy: Investigating Subinhibitory Effects of Amikacin on Pseudomonas aeruginosa

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• 作者：E. Consuelo Ló ; pez-Dí ; ez ; Catherine L. Winder ; Lorna Ashton ; Felicity Currie ; and Royston Goodacre
• 刊名：Analytical Chemistry
• 出版年：2005
• 出版时间：May 1, 2005
• 年：2005
• 卷：77
• 期：9
• 页码：2901 - 2906
• 全文大小：226K
• 年卷期：v.77,no.9(May 1, 2005)
• ISSN：1520-6882

文摘

During the last 20 years the rate at which new antimicrobial agents are produced has decreased dramatically, withconcomitant increase in the number of pathogens that arebecoming multidrug resistant. Together these have created a patient healthcare risk and this is of great concern.A crucial aspect for the discovery of new antibiotics is thedevelopment of new techniques that allow rapid andaccurate characterization of the mode of action of thepharmacophore. In this work UV resonance Raman(UVRR) spectroscopy has been developed to monitor theconcentration effect of antibiotics on bacterial cells. UVRRwas conducted at 244 nm and spectra were collected intypically 60 s. Supervised multivariate analysis and 2Dcorrelation spectroscopy were used to evaluate whetherthe UVRR spectra contained valuable information thatcould be used to study the mode of action of antibiotics.The clustering pattern in the discriminant factors spacecorrelated directly to the concentration of amikacin, andpartial least squares (PLS) regression analysis of theUVRR spectra was able to predict the concentration ofamikacin to which bacterial cells had been exposed. 2Dcorrelation spectroscopy contour maps indicated thatspectral changes due to the presence of amikacin in thegrowth media occur according to the known mode ofaction of the studied antibiotic. Therefore, we concludethat UVRR spectroscopy, when coupled with chemometrics and 2D correlation spectroscopy, constitutes a powerful approach for the development and screening of newantibiotics.