Self-Assembly-Assisted Biomolecule-Enriched Surface and High Selectivity Performance of Simple Solution-Coatable Biomimicking Brush Copolymers

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• 作者：Kyungho Kwon ; Changsub Kim ; Jongchan Lee ; Heesoo Kim ; Moonhor Ree
• 刊名：Biomacromolecules
• 出版年：2016
• 出版时间：March 14, 2016
• 年：2016
• 卷：17
• 期：3
• 页码：974-984
• 全文大小：571K
• ISSN：1526-4602

文摘

Poly(oxy(11-(biotinyl)undecylthiomethyl)ethylene-co-oxy(11-phosphoryl-cholineundecylthiomethyl)ethylene)s (PECH-BT_mPC_n: m = 0–100 mol % biotin (BT)-containing bristle; n = 100–0 mol % phosphorylcholine (PC)-containing bristle) were newly synthesized. All polymers exhibited excellent solution processability. They favorably self-assembled horizontal multibilayer structures in thin films with BT- and PC-enriched surfaces, which were driven by the lateral ordering of the fully extended upright bristles and the partial interdigitation between the BT and PC end groups of the bristles. Both hydrophilicity and water sorption of the films increased with the PC content. The PECH-BT₁₀₀ films revealed remarkably distinctive sensitivity, selectivity, and adsorption ability for avidin against other proteins. Such remarkable performance was further significantly enhanced on the PECH-BT_mPC_n films in which PC moieties were incorporated to the BT-rich surface; in particular, the PECH-BT₇₅PC₂₅ films demonstrated the highest performance. Overall, the self-assembly brush copolymers of this study are very suitable for use in the high performance detection, adsorption, and separation of proteins and receptors, including avidin, which can reveal high affinity and selectivity to BT moiety.