Sulfobetaine鈥揤inylimidazole Block Copolymers: A Robust Quantum Dot Surface Chemistry Expanding Bioimaging鈥檚 Horizons

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• 作者：Mariana Tasso ; Emerson Giovanelli ; Diana Zala ; Sophie Bouccara ; Alexandra Fragola ; Mohamed Hanafi ; Zsolt Lenkei ; Thomas Pons ; Nicolas Lequeux
• 刊名：ACS Nano
• 出版年：2015
• 出版时间：November 24, 2015
• 年：2015
• 卷：9
• 期：11
• 页码：11479-11489
• 全文大小：602K
• ISSN：1936-086X

文摘

Long-term inspection of biological phenomena requires probes of elevated intra- and extracellular stability and target biospecificity. The high fluorescence and photostability of quantum dot (QD) nanoparticles contributed to foster their promise as bioimaging tools that could overcome limitations associated with traditional fluorophores. However, QDs鈥?potential as a bioimaging platform relies upon a precise control over the surface chemistry modifications of these nano-objects. Here, a zwitterion鈥搗inylimidazole block copolymer ligand was synthesized, which regroups all anchoring groups in one compact terminal block, while the rest of the chain is endowed with antifouling and bioconjugation moieties. By further application of an oriented bioconjugation approach with whole IgG antibodies, QD nanobioconjugates were obtained that display outstanding intra- and extracellular stability as well as biorecognition capacity. Imaging the internalization and intracellular dynamics of a transmembrane cell receptor, the CB1 brain cannabinoid receptor, both in HEK293 cells and in neurons, illustrates the breadth of potential applications of these nanoprobes.