Self-assembled Protein Arrays from an Ornithodoros moubata Salivary Gland Expression Library

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• 作者：Raul Manzano-Rom谩n ; Veronica D铆az-Mart铆n ; Maria Gonz谩lez-Gonz谩lez ; Sergio Matarraz ; Angel Francisco 脕lvarez-Prado ; Joshua LaBaer ; Alberto Orfao ; Ricardo P茅rez-S谩nchez ; Manuel Fuentes
• 刊名：Journal of Proteome Research
• 出版年：2012
• 出版时间：December 7, 2012
• 年：2012
• 卷：11
• 期：12
• 页码：5972-5982
• 全文大小：434K
• 年卷期：v.11,no.12(December 7, 2012)
• ISSN：1535-3907

文摘

Protein interactions play a critical role in the regulation of many biological events and their study in a high-throughput format has become a key area of proteomic research. Nucleid Acid Programmable Protein Arrays (NAPPA) technology allows the construction of protein arrays from cDNA expression libraries in high-throughput cell-free systems to study protein interaction and functions. Tick saliva contains antihemostatic, anti-inflammatory, and immunosuppressive proteins that counteract the host hemostatic, immune, and inflammatory responses allowing the ingestion of host blood and facilitating its infection by the tick-borne pathogens. Identification of such proteins and their functions could help in the selection of antigenic targets for the development of antitick and transmission-blocking vaccines. With that aim, we have prepared a cDNA expression library from the salivary glands of Ornithodoros moubata and subsequently produced a self-assembled protein microarray using 480 randomly selected clones from that library. The reproducibility of the array, its representativeness of the tick salivary protein repertoire, and the functionality of the in situ expressed proteins have been checked, demonstrating that it is a suitable tool for the identification and functional characterization of soft tick salivary molecules that interact with host proteins. Several clones in the array were shown to bind to human recombinant P-selectin. One of them was a likely secreted tick phospholipase A2, which may represent a potential new ligand for P-selectin. As these salivary molecules are likely involved in blood meal acquisition through the modulation of the host immune and hemostatic responses, this new high-throughput tool could open new avenues for development of new therapeutic agents and control strategies against ticks and tick-borne pathogens.

Keywords:

protein arrays; ticks&qsSearchArea=searchText">ticks; salivary glands; expression libraries; interactions