Quantitation of Silibinin, a Putative Cancer Chemopreventive Agent Derived from Milk Thistle (Silybum marianum), in Human Plasma by High-Performance Liquid Chromatography and Identification of
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- 作者：Carmen S. L. Hoh ; David J. Boocock ; Timothy H. Marczylo ; V. A. Brown ; Hong Cai ; William P. Steward ; David P. Berry ; and ; Andreas J. Gescher
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：April 4, 2007
- 年：2007
- 卷：55
- 期：7
- 页码：2532 - 2535
- 全文大小：50K
- 年卷期：v.55,no.7(April 4, 2007)
- ISSN：1520-5118
文摘
Silibinin has recently received attention as a potential cancer chemopreventive agent because of itsantiproliferative and anticarcinogenic effects. A simple and specific reversed-phase high-performanceliquid chromatography method was developed and validated for the quantitation of silibinin in humanplasma. Sample preparation involved simple protein precipitation, and separation was achieved ona Waters Atlantis C18 column with flow rate of 1.0 mL/min at 40 
deg.gif">C and UV detection at 290 nm.Silibinin was detected as two peaks corresponding to trans-diastereoisomers. The peak area waslinear over the investigated concentration range (0-5000 ng/mL). The limits of detection were 2 and1 ng/mL for the two diastereoisomers (d1 and d2), with a recovery of 53-58%. This method wasutilized to detect silibinin in plasma of colorectal patients after 7 days of treatment with silipide (silibininformulated with phosphatidyl choline).
deg.gif">C and UV detection at 290 nm.Silibinin was detected as two peaks corresponding to trans-diastereoisomers. The peak area waslinear over the investigated concentration range (0-5000 ng/mL). The limits of detection were 2 and1 ng/mL for the two diastereoisomers (d1 and d2), with a recovery of 53-58%. This method wasutilized to detect silibinin in plasma of colorectal patients after 7 days of treatment with silipide (silibininformulated with phosphatidyl choline).