Synthesis of Triazole-Linked Oligonucleotides with High Affinity to DNA Complements and an Analysis of Their Compatibility with Biosystems

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• 作者：Anna M. Varizhuk ; Dmitry N. Kaluzhny ; Roman A. Novikov ; Alexandr O. Chizhov ; Igor P. Smirnov ; Andrey N. Chuvilin ; Olga N. Tatarinova ; Gleb Y. Fisunov ; Galina E. Pozmogova ; Vladimir L. Florentiev
• 刊名：The Journal of Organic Chemistry
• 出版年：2013
• 出版时间：June 21, 2013
• 年：2013
• 卷：78
• 期：12
• 页码：5964-5969
• 全文大小：292K
• 年卷期：v.78,no.12(June 21, 2013)
• ISSN：1520-6904

文摘

New oligonucleotide analogues with triazole internucleotide linkages were synthesized, and their hybridization properties were studied. The analogues demonstrated DNA binding affinities similar to those of unmodified oligonucleotides. The modification was shown to protect the oligonucleotides from nuclease hydrolysis. The modified oligonucleotides were tested as PCR primers. Modifications remote from the 3鈥?terminus were tolerated by polymerases. Our results suggest that these new oligonucleotide analogues are among the most promising triazole DNA mimics characterized to date.