Three Novel Alkaloids from Portulaca oleracea L. and Their Anti-inflammatory Effects

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• 作者：Cui-Yu Li ; Yi-Han Meng ; Zhe-Ming Ying ; Nan Xu ; Dong Hao ; Ming-Zhe Gao ; Wen-Jie Zhang ; Liang Xu ; Yu-Cong Gao ; Xi-Xiang Ying
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：July 27, 2016
• 年：2016
• 卷：64
• 期：29
• 页码：5837-5844
• 全文大小：433K
• 年卷期：0
• ISSN：1520-5118

文摘

Three novel carbon skeleton alkaloids, named oleracimine (1), oleracimine A (2), and oleracone A (3), with one novel azulene carbon skeleton compound, oleracone B (4), and one known compound, β-carboline (5), were first isolated from Portulaca oleracea L. The structures were determined using spectroscopic methods, including one- and two-dimensional nuclear magnetic resonance and high-resolution electrospray ionization time-of-flight mass spectrometry techniques. In addition, oleracimine (1) was used to investigate the anti-inflammatory effects on lipopolysaccharide-stimulated macrophages. The results of enzyme-linked immunosorbent assay, western blot, and real-time polymerase chain reaction showed that oleracimine (1) remarkably inhibited nitric oxide production and could dose-dependently decrease the secretions of interleukin 6, tumor necrosis factor α, nitric oxide, and prostaglandin E₂ in cell culture supernatants as well as the mRNA of cyclooxygenase-2 and inducible nitric oxide synthase.