LuxS-Dependent AI-2 Regulates Versatile Functions in Enterococcus faecalis V583
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- 作者：Changlin Shao ; Wei Shang ; Zhan Yang ; Zhongke Sun ; Yunmei Li ; Jing Guo ; Xuesong Wang ; DaYang Zou ; Simiao Wang ; Hong Lei ; Qian Cui ; Zhitao Yin ; Xuelian Li ; Xiao Wei ; Wei Liu ; Xiang He ; Zheng Jiang ; Shuangkui Du ; Xiangru Liao ; Liuyu Huang ; Yufei Wang ; Jing Yuan
- 刊名：Journal of Proteome Research
- 出版年：2012
- 出版时间：September 7, 2012
- 年：2012
- 卷：11
- 期：9
- 页码：4465-4475
- 全文大小：407K
- 年卷期：v.11,no.9(September 7, 2012)
- ISSN：1535-3907
文摘
Bacteria utilize a quorum sensing (QS) system to coordinate gene expression by monitoring the concentration of molecules known as autoinducers (AI). In the present study, we confirmed the presence of a LuxS/AI-2 dependent QS system in vancomycin-resistant Enterococcus faecalis V583. Then, the cellular targets controlled by AI-2 were identified by comparative proteomics analysis in order to elucidate the possible role of AI-2 in E. faecalis. Results demonstrated 15 proteins that are differentially expressed upon the addition of AI-2, including proteins involved in metabolism, translation, energy production and/or conversion, and cell wall biogenesis. Glyceraldehyde-3-phosphate dehydrogenase and malate dehydrogenase associated with carbohydrate metabolism and energy production were up-regulated upon inducing by AI-2. In addition, externally added AI-2 could down-regulate acetyl-coenzyme A carboxylase and ornithine carbamoyltransferase, two key enzyme involved in metabolism. All these data suggest that AI-2 signaling may play a role in the regulation of a number of important metabolic properties of E. faecali. We further investigated the role of AI-2 in biofilm formation by E. faecalis, showing the addition of AI-2 to E. faecalis V583 cultures resulted in increased biofilm formation. Our results provide important clues to the role of a LuxS/AI-2 dependent QS system in vancomycin-resistant E. faecalis.