iTRAQ-Based Proteomics Revealed the Bactericidal Mechanism of Sodium New Houttuyfonate against Streptococcus pneumoniae

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• 作者：Xiao-Yan Yang ; Tianyuan Shi ; Gaofei Du ; Wanting Liu ; Xing-Feng Yin ; Xuesong Sun ; Yunlong Pan ; Qing-Yu He
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：August 17, 2016
• 年：2016
• 卷：64
• 期：32
• 页码：6375-6382
• 全文大小：471K
• 年卷期：0
• ISSN：1520-5118

文摘

Sodium new houttuyfonate (SNH), an addition product of active ingredient houttuynin from the plant Houttuynia cordata Thunb., inhibits a variety of bacteria, yet the mechanism by which it induces cell death has not been fully understood. In the present study, we utilized iTRAQ-based quantitative proteomics to analyze the protein alterations in Streptococcus pneumoniae in response to SNH treatment. Numerous proteins related to the production of reactive oxygen species (ROS) were found to be up-regulated by SNH, suggesting that ROS pathways may be involved as analyzed via bioinformatics. As reported recently, cellular reactions stimulated by ROS including superoxide anion (O₂^•–), hydrogen peroxide (H₂O₂), and hydroxyl radicals (OH^•) have been implicated as mechanisms whereby bactericidal antibiotics kill bacteria. We then validated that SNH killed S. pneumoniae in a dose-dependent manner accompanied by the increasing level of H₂O₂. On the other hand, the addition of catalase, which can neutralize H₂O₂ in cells, showed a significant recovery in bacterial survival. These results indicate that SNH indeed induced H₂O₂ formation to contribute to the cell lethality, providing new insights into the bactericidal mechanism of SNH and expanding our understanding of the common mechanism of killing induced by bactericidal agents.