Event-Specific Detection of Seven Genetically Modified Soybean and Maizes Using Multiplex-PCR Coupled with Oligonucleotide Microarray

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• 作者：Jia Xu ; Shuifang Zhu ; Haizhen Miao ; Wensheng Huang ; Minyan Qiu ; Yan Huang ; Xuping Fu ; Yao Li
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2007
• 出版时间：July 11, 2007
• 年：2007
• 卷：55
• 期：14
• 页码：5575 - 5579
• 全文大小：101K
• 年卷期：v.55,no.14(July 11, 2007)
• ISSN：1520-5118

文摘

With the increasing development of genetically modified organism (GMO) detection techniques, thepolymerase chain reaction (PCR) technique has been the mainstay for GMO detection. Anoligonucleotide microarray is a glass chip to the surface of which an array of oligonucleotides wasfixed as spots, each containing numerous copies of a sequence-specific probe that is complementaryto a gene of interest. So it is used to detect ten or more targets synchronously. In this research, anevent-specific detection strategy based on the unique and specific integration junction sequencesbetween the host plant genome DNA and the integrated gene is being developed for its high specificityusing multiplex-PCR together with oligonucleotide microarray. A commercial GM soybean (GTS 40-3-2) and six GM maize events (MON810, MON863, Bt176, Bt11, GA21, and T25) were detected bythis method. The results indicate that it is a suitable method for the identification of these GM soybeanand maizes.