Ag@Poly(m-phenylenediamine) Core鈭扴hell Nanoparticles for Highly Selective, Multiplex Nucleic Acid Detection

详细信息    查看全文

• 作者：Yingwei Zhang ; Lei Wang ; Jingqi Tian ; Hailong Li ; Yonglan Luo ; Xuping Sun
• 刊名：Langmuir
• 出版年：2011
• 出版时间：March 15, 2011
• 年：2011
• 卷：27
• 期：6
• 页码：2170-2175
• 全文大小：895K
• 年卷期：v.27,no.6(March 15, 2011)
• ISSN：1520-5827

文摘

In this letter, we report on the one-step synthesis of Ag@poly(m-phenylenediamine) core鈭抯hell nanoparticles (APCSNPs), carried out by direct mixing of aqueous silver nitrate and m-phenylenediamine solutions at room temperature. We further demonstrate the use of APCSNP as a novel fluorescent sensing platform for nucleic acid detection. In this regard, the detection of DNA is accomplished in two steps. First, APCSNP absorbs and quenches the fluorescence of dye-labeled single-stranded DNA (ssDNA) as a probe. Second, hybridizing of the probe with its target produces a double-stranded DNA (dsDNA) that detaches from APCSNP, resulting in the recovery of dye fluorescence. It suggests that this sensing system has a high selectivity down to single-base mismatch, and the results exhibit good reproducibility. Furthermore, we also demonstrate its application for the multiplex detection of nucleic acid sequences.