Poly(o-phenylenediamine) Colloid-Quenched Fluorescent Oligonucleotide as a Probe for Fluorescence-Enhanced Nucleic Acid Detection

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• 作者：Jingqi Tian ; Hailong Li ; Yonglan Luo ; Lei Wang ; Yingwei Zhang ; Xuping Sun
• 刊名：Langmuir
• 出版年：2011
• 出版时间：February 1, 2011
• 年：2011
• 卷：27
• 期：3
• 页码：874-877
• 全文大小：752K
• 年卷期：v.27,no.3(February 1, 2011)
• ISSN：1520-5827

文摘

In this Letter, we demonstrate that chemical oxidation polymerization of o-phenylenediamine (OPD) by potassium bichromate at room temperature results in the formation of submicrometer-scale poly(o-phenylenediamine) (POPD) colloids. Such colloids can absorb and quench dye-labeled single-stranded DNA (ssDNA) very effectively. In the presence of a target, a hybridization event occurs, which produces a double-stranded DNA (dsDNA) that detaches from the POPD surface, leading to recovery of dye fluorescence. With the use of an oligonucleotide (OND) sequence associated with human immunodeficiency virus (HIV) as a model system, we demonstrate the proof of concept that POPD colloid-quenched fluorescent OND can be used as a probe for fluorescence-enhanced nucleic acid detection with selectivity down to single-base mismatch.