Modified Enzyme-Linked Immunosorbent Assay Strategy Using Graphene Oxide Sheets and Gold Nanoparticles Functionalized with Different Antibody Types

详细信息    查看全文

• 作者：Hongjun Lin ; Yingfu Liu ; Jingrui Huo ; Aihong Zhang ; Yiting Pan ; Haihong Bai ; Zhang Jiao ; Tian Fang ; Xin Wang ; Yun Cai ; Qingming Wang ; Yangjun Zhang ; Xiaohong Qian
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：July 2, 2013
• 年：2013
• 卷：85
• 期：13
• 页码：6228-6232
• 全文大小：241K
• 年卷期：v.85,no.13(July 2, 2013)
• ISSN：1520-6882

文摘

Gold nanoparticles (GNPs) and graphene oxide (GO) sheets are excellent nano carriers in many analytical methods. In this study, a modified enzyme-linked immunosorbent assay (ELISA) strategy was developed using antibody-functionalized GO sheets and GNPs. This modification significantly reduced the limit of detection (LOD) and cost greatly of this assay. The applicability of the method was demonstrated by detecting HSP70 in a human serum sample. This result suggests that the 3G-ELISA method is feasible to detect an antigen in a complex mixture, and the LOD is up to 64-fold and the cost is as low as one-tenth of the conventional ELISA method.