Molecular Logic Gates on DNA Origami Nanostructures for MicroRNA Diagnostics

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• 作者：Dongfang Wang ; Yanming Fu ; Juan Yan ; Bin Zhao ; Bin Dai ; Jie Chao ; Huajie Liu ; Dannong He ; Yi Zhang ; Chunhai Fan ; Shiping Song
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：February 18, 2014
• 年：2014
• 卷：86
• 期：4
• 页码：1932-1936
• 全文大小：257K
• ISSN：1520-6882

文摘

Molecular computing holds great promise for diagnosis and treatment of diseases at the molecular level; nevertheless, designing molecular logic gates to operate programmably and autonomously for molecular diagnostics still remains challenging. We designed logic gates on DNA Origami for microRNA analysis. As a demonstration, two indicators of heart failure, microRNA-21 and microRNA-195, were selected as the logic inputs. The logic gates contain two main modules: computation module and output module, performing in a single DNA Origami nanostructure. The computation module recognizes disease indicators, while the output module display different nanoscale symbols, 鈥?鈥?(positive) or 鈥溾€撯€?(negative), depending on the computing results. We demonstrated that the molecular logic gates worked well with single and two input combinations.