Peptide-Assisted Directional Adsorption of Purple Membrane at the Liquid鈥揝olid Interface

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• 作者：Huiwen Du ; Denghua Li ; Yibing Wang ; Chenxuan Wang ; Ping Li ; Yan-lian Yang ; Chen Wang
• 刊名：Journal of Physical Chemistry C
• 出版年：2014
• 出版时间：December 26, 2014
• 年：2014
• 卷：118
• 期：51
• 页码：29770-29776
• 全文大小：433K
• ISSN：1932-7455

文摘

We report here an approach of directional adsorption of purple membrane (PM) on liquid鈥搒olid interfaces modified by peptide assemblies. Bacteriorhodopsin (bR) is the only protein in PM that can transport protons directionally from the cytoplasmic (CP) side to the extracellular (EC) side to achieve chemical energy for life and growth. Controlled adsorption of PM is critical to exploring novel properties in many areas, such as data storage, biosolar devices, and sensors. Here, we obtained oriented PM adsorption at the liquid鈥搒olid interface by modification with de novo peptides. EFM was utilized to distinguish the two sides of PM by measuring the surface potential of PM because of its high resolution in differentiating electrical characteristics. Furthermore, we confirmed the modulating effect by photoelectrical responses under laser irradiation.