Cytotoxic Constituents from Lobaria scrobiculata and a Comparison of Two Bioassays for Their Evaluation
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- 作者：Andreas Schinkovitz ; Aman Kaur ; Ernst Urban ; Martin Zehl ; Gabriela P谩chnikov谩 ; Yuehong Wang ; Nadine Kretschmer ; Iva Slaninov谩 ; Guido F. Pauli ; Scott G. Franzblau ; Georg Krupitza ; Rudolf Bauer ; Brigitte Kopp
- 刊名：Journal of Natural Products
- 出版年：2014
- 出版时间：April 25, 2014
- 年：2014
- 卷：77
- 期：4
- 页码：1069-1073
- 全文大小：274K
- 年卷期：v.77,no.4(April 25, 2014)
- ISSN：1520-6025
文摘
Lichens are resilient organisms, known for their unique profiles of secondary metabolites and for exhibiting antioxidative, antibacterial, and cytotoxic effects. Analyzing the cytotoxic potential of Lobaria scrobiculata, a bioassay-guided fractionation strategy yielded seven known metabolites, with two of these compounds, 2 and 3, exhibiting cytotoxicity against HL-60 cells. In order to verify the potential impact of degradation on observed bioactivity, a purity and stability evaluation was conducted. The consistency of results obtained by the water-soluble tetrazolium salt-1 assay and trypan blue cytotoxicity assay was evaluated for selected compounds.