Automated Multiplug Filtration Cleanup for Pesticide Residue Analyses in Kiwi Fruit (Actinidia chinensis) and Kiwi Juice by Gas Chromatography–Mass Spectrometry

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• 作者：Yuhong Qin ; Jingru Zhang ; Yining He ; Yongtao Han ; Nan Zou ; Yanjie Li ; Ronghua Chen ; Xuesheng Li ; Canping Pan
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：August 10, 2016
• 年：2016
• 卷：64
• 期：31
• 页码：6082-6090
• 全文大小：327K
• 年卷期：0
• ISSN：1520-5118

文摘

To reduce labor-consuming manual operation workload in the cleanup steps, an automated multiplug filtration cleanup (m-PFC) method for QuEChERS (quick, easy, cheap, effective, rugged, and safe) extracts was developed. It could control the volume and speed of pulling and pushing cycles accurately. In this study, m-PFC was based on multiwalled carbon nanotubes (MWCNTs) mixed with primary–secondary amines (PSA) and anhydrous magnesium sulfate (MgSO₄) in a packed column for analysis of pesticide residues followed by gas chromatography–mass spectrometry (GC-MS) detection. It was validated by analyzing 33 pesticides in kiwi fruit and kiwi juice matrices spiked at two concentration levels of 10 and 100 μg/kg. Salts, sorbents, m-PFC procedure, 4 mL of automated pulling and pushing volume, 6 mL/min automated pulling speed, and 8 mL/min pushing speed were optimized for each matrix. After optimization, spike recoveries were within 71–120% and <20% RSD for all analytes in kiwi fruit and kiwi juice. Matrix-matched calibrations were performed with the coefficients of determination >0.99 between concentration levels of 10 and 1000 μg/kg. The developed method was successfully applied to the determination of pesticide residues in market samples.