Characterization and Modeling of Transcriptional Cross-Regulation in Acinetobacter baylyi ADP1
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- 作者：Dayi Zhang ; Yun Zhao ; Yi He ; Yun Wang ; Yiyu Zhao ; Yi Zheng ; Xia Wei ; Litong Zhang ; Yuzhen Li ; Tao Jin ; Lin Wu ; Hui Wang ; Paul A. Davison ; Junguang Xu ; Wei E. Huang
- 刊名：ACS Synthetic Biology
- 出版年：2012
- 出版时间：July 20, 2012
- 年：2012
- 卷：1
- 期：7
- 页码：274-283
- 全文大小：475K
- 年卷期：v.1,no.7(July 20, 2012)
- ISSN：2161-5063
文摘
Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation of novel tasks. Transcriptional gene regulation systems induced by small molecules in prokaryotes provide a rich source for logic gates. Cross-regulation, whereby a promoter is activated by different molecules or different promoters are activated by one molecule, can be used to design an OR-gate and achieve cross-talk between gene networks in cells. Acinetobacter baylyi ADP1 is naturally transformable, readily editing its chromosomal DNA, which makes it a convenient chassis for synthetic biology. The catabolic genes for salicylate, benzoate, and catechol metabolism are located within a supraoperonic cluster (-sal-are-ben-cat-) in the chromosome of A. baylyi ADP1, which are separately regulated by LysR-type transcriptional regulators (LTTRs). ADP1-based biosensors were constructed in which salA, benA, and catB were fused with a reporter gene cassette luxCDABE under the separate control of SalR, BenM, and CatM regulators. Salicylate, benzoate, catechol, and associated metabolites were found to mediate cross-regulation among sal, ben, and cat operons. A new mathematical model was developed by considering regulator-inducer binding and promoter activation as two separate steps. This model fits the experimental data well and is shown to predict cross-regulation performance.