Quantitative Assessment of Protein Adsorption on Microparticles with Particle Mass Spectrometry

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• 作者：Caiqiao Xiong ; Xiaoyu Zhou ; Ning Zhang ; Lingpeng Zhan ; Suming Chen ; Jiyun Wang ; Wen-Ping Peng ; Huan-Cheng Chang ; Zongxiu Nie
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：April 15, 2014
• 年：2014
• 卷：86
• 期：8
• 页码：3876-3881
• 全文大小：307K
• 年卷期：v.86,no.8(April 15, 2014)
• ISSN：1520-6882

文摘

In this paper, particle mass spectrometry (PMS), which consists of an aerodynamic desorption/ionization (AD) source, a quadrupole ion trap (QIT) mass analyzer, and a charge detector, was exploited to characterize the protein adsorption on microparticles based on the mass variations of microparticles before and after protein adsorption. This method is simple and has low sample cost. Importantly, its mass resolution is good enough to distinguish the microparticles with and without protein. For the adsorption of bovine serum albumin (BSA) on 3 渭m porous poly styrene-divinylbenzene (poly S-DVB), the minimum mass increase that can be resolved by PMS corresponds to 128 fg (1.8 ng/cm²) or 1.17 脳 10⁶ BSA molecules on each poly S-DVB particle. With PMS, the adsorption process of BSA on poly S-DVB spheres was successfully characterized, and the obtained maximum adsorption capacity q_m and dissociation constant K_d were consistent with that determined by the conventional depletion method. In addition, the influence of surface modification of silica particles on the enzyme immobilization was evaluated. Compared with C₄ (propyldimethylsilane), C₈ (octyldimethylsilane), and Ph (phenyldimethylchlorosilane), the CN (cyanoethyldimethylchlorosilane) functionalized silica particles were screened to be most beneficial for the immobilization of both lysozyme and trypsin.