Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity

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• 作者：Robert Y. Henley ; Brian Alan Ashcroft ; Ian Farrell ; Barry S. Cooperman ; Stuart M. Lindsay ; Meni Wanunu
• 刊名：Nano Letters
• 出版年：2016
• 出版时间：January 13, 2016
• 年：2016
• 卷：16
• 期：1
• 页码：138-144
• 全文大小：491K
• ISSN：1530-6992

文摘

It has been hypothesized that the ribosome gains additional fidelity during protein translation by probing structural differences in tRNA species. We measure the translocation kinetics of different tRNA species through ～3 nm diameter synthetic nanopores. Each tRNA species varies in the time scale with which it is deformed from equilibrium, as in the translocation step of protein translation. Using machine-learning algorithms, we can differentiate among five tRNA species, analyze the ratios of tRNA binary mixtures, and distinguish tRNA isoacceptors.