Isolation and characterization of bioactive fungi from shark Carcharodon carcharias' gill with biopharmaceutical prospects

详细信息    查看全文

• 作者：Yi Zhang 张翼 ; Jinyuan Han 韩金娿/a> ; Yan Feng 冯妍…
• 关键词：bioactive fungi ; shark gill ; isolation ; bioassay ; taxonomy ; bioactive metabolites
• 刊名：Chinese Journal of Oceanology and Limnology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：34
• 期：1
• 页码：186-199
• 全文大小：1,168 KB
• 参考文献：Abbott W S. 1925. A method of computing the effectiveness of an insecticide. J. Econ. Entomol., 18 (2): 265–267.CrossRef
  Alam M Z, Ahmad S, Malik A. 2009. Genotoxic and mutagenic potential of agricultural soil irrigated with tannery effluents at Jajmau (Kanpur), India. Arch. Environ. Contam. Toxicol., 57 (3): 463–476.CrossRef
  Armstrong E, Yan L M, Boyd K G, Wright P C, Burgess J G. 2001. The symbiotic role of marine microbes on living surfaces. Hydrobiologia, 461 (1-3): 37–40.CrossRef
  Baker P W, Kennedy J, Dobson A D, Marchesi J R. 2009. Phylogenetic diversity and antimicrobial activities of fungi associated with Haliclona simulans isolated from Irish coastal waters. Mar. Biotechnol., 11 (4): 540–547.CrossRef
  Bartus H R, Mirabelli C K, Auerbach J I, Shatzman A R, Taylor D P, Johnson R K, Rosenberg M, Crooke S T. 1984. Improved genetically modified Escherichia coli strain for prescreening antineoplastic agents. Antimicrob. Agents. Chemother., 25 (5): 622–625.CrossRef
  Blunt J W, Copp B R, Munro M H, Northcote P T, Prinsep M R. 2011. Marine natural products. Nat. Prod. Rep., 28 (2): 196–268.CrossRef
  Buckingham J. 2011. Dictionary of Natural Products on DVD. version 20: 2. Chapman and Hall/CRC, London.
  Bugni T S, Ireland C M. 2004. Marine-derived fungi: a chemically and biologically diverse group of microorganisms. Nat. Prod. Rep., 21 (1): 143–163.CrossRef
  Carballo J L, Herná ndez-Inda Z L, Pérez P, García-Grávalos M D. 2002. A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products. BMC Biotechnol., 2: 17.CrossRef
  De Maddalena A. 2007. Great White Sharks Preserved in European Museums. Cambridge Scholars Publishing, Newcastle.
  Ding B, Yin Y, Zhang F L, Li Z Y. 2011. Recovery and phylogenetic diversity of culturable fungi associated with marine sponges Clathrina luteoculcitella and Holoxea sp. in the South China Sea. Mar. Biotechnol., 13 (4): 713–721.CrossRef
  Du L, Feng T, Zhao B Y, Li D H, Cai S X, Zhu T J, Wang F P, Xiao X, Gu Q Q. 2010. Alkaloids from a deep ocean sediment-derived fungus Penicillium sp. and their antitumor activities. J. Antibiot. ( Tokyo ), 63 (4): 165–170.CrossRef
  Hentschel U, Piel J, Degnan S M, Taylor M W. 2012. Genomic insights into the marine sponge microbiome. Nat. Rev. Microbiol., 10 (9): 641–654.CrossRef
  Hu Z Y, Liu S Z, Huang H, Huang C S, Su W J. 2000. A rapid screening assay for insecticidal antibiotic produced by marine actinomycetes. Mar. Sci. Bul. ( Haiyang Tongbao ), 19 (4): 36–41. (in Chinese with English abstract)
  Knickle C, Billingsley L, DiVittorio K. 2011. Biological profiles basking shark. Florida Museum of Natural History. http://​www.​flmnh.​ufl.​edu/​fish/​Gallery/​Descript/​ baskingshark/baskingshark.html. Retrieved 2011-12-21.
  Kobayashi H, Namikoshi M, Yoshimoto T, Yokochi T. 1996. A screening method for antimitotic and antifungal substances using conidia of Pyricularia oryzae, modification and application to tropical marine fungi. J. Antibiot. ( Tokyo ), 49 (9): 873–879.CrossRef
  Kobayashi J, Ishibashi M. 1993. Bioactive metabolites of symbiotic marine microorganisms. Chem. Rev., 93 (5): 1 753–1 769.
  Li G, Lin Y. 2004. Differential DNA repair test for screening of the anti-tumor marine microorganisms. Chin. J. Antibiot. ( Zhongguo Kangshengsu Zazhi ), 29 (8): 449–451. (in Chinese with English abstract)
  Menezes C B A, Bonugli-Santos R C, Miqueletto P B, Passarini M R Z, Silva C H, Justo M R, Leal R R, Fantinatti-Garboggini F, Oliveira V M, Berlinck R G S, Sette L D. 2010. Microbial diversity associated with algae, ascidians and sponges from the north coast of São Paulo state, Brazil. Microbiol. Res., 165 (6): 466–482.CrossRef
  Meng Q W, Su J X, Li W D. 1987. Comparative Anatomy of Fish. Science Press, Beijing. (in Chinese)
  Mohammed Y S, Luckner M. 1963. The structure of cyclopenin and cyclopenol, metabolic products from P enicillium cyclopium westling and P enicillium viridicatum westling. Tetrahedron Lett., 4 (28): 1 953–1 958.CrossRef
  Mueller H, Kassack M U, Wiese M. 2004. Comparison of the usefulness of the MTT, ATP, and calcein assays to predict the potency of cytotoxic agents in various human cancer cell lines. J. Biomol. Screen., 9 (6): 506–515.CrossRef
  Passarini M R Z, Santos C, Lima N, Berlinck R G S, Sette L D. 2013. Filamentous fungi from the Atlantic marine sponge Dragmacidon reticulatum. Arch. Microbiol., 195 (2): 99–111.CrossRef
  Peterson S W. 2012. Aspergillus and Penicillium identification using DNA sequences: barcode or MLST? Appl. Microbiol. Biotechnol., 95 (2): 339–344.CrossRef
  Pieter S S, Robert V. 1983. Roquefortine, an intermediate in the biosynthesis of oxaline in cultures of Penicillium oxalicum. J. Chem. Soc., Chem. Comm., (10): 560–561.
  Pinkerton F, Strobel G. 1976. Serinol as an activator of toxin production in attenuated cultures of Helminthosporium sacchari. Proc. Natl. Acad. Sci. U. S. A., 73 (11): 4 007–4 011.CrossRef
  Proksch P, Putz A, Ortlepp S, Kjer J, Bayer M. 2010. Bioactive natural products from marine sponges and fungal endophytes. Phytochem. Rev., 9 (4): 475–489.CrossRef
  Raghukumar C. 2008. Marine fungal biotechnology: an ecological perspective. Fungal Divers., 31: 19–35.
  Strobel G, Yang X, Sears J, Kramer R, Sidhu R S, Hess W M. 1996. Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana. Microbiology, 142 (2): 435–440.CrossRef
  Swathi J, Narendra K, Sowjanya K M, Satya A K. 2013. Marine fungal metabolites as a rich source of bioactive compounds. Afr. J. Biochem. Res., 7 (10): 184–196.CrossRef
  Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol., 24 (8): 1 596–1 599.CrossRef
  Thompson J D, Gibson T J, Plewniak F, Jeanmougin F, Higgins D G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic. Acids. Res., 25 (24): 4 876–4 882.CrossRef
  White T J, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M A G D, Sninsky J J, White T J eds. Pcr Protocols: A Guide to Methods and Applications. 1 st edn. Academic Press, New York. p.315–322.
  Wiese J, Ohlendorf B, Blümel M, Schmaljohann R, Imhoff J F. 2011. Phylogenetic identification of fungi isolated from the marine sponge Tethya aurantium and identification of their secondary metabolites. Mar. Drugs, 9 (4): 561–585.CrossRef
  Xin Z H, Fang Y, Du L, Zhu T, Duan L, Chen J, Gu Q Q, Zhu W M. 2007. Aurantiomides A-C, quinazoline alkaloids from the sponge-derived fungus Penicillium aurantiogriseum SP0-19. J. Nat. Prod., 70 (5): 853–855.CrossRef
  Xiong L, Li J, Kong F. 2004. Streptomyces sp. 173, an insecticidal micro-organism from marine. Lett. Appl. Microbiol., 38 (1): 32–37.
  Xu Y J, Yan X J. 2006. Research advances in chemical ecology of marine microorganisms. Chin. J. Appl. Ecol., 17 (12): 2 436–2 440. (in Chinese with English abstract)
  Zhang Y, Mu J, Han J, Gu X. 2012. An improved brine shrimp larvae lethality microwell test method. Toxicol. Mech. Methods., 22 (1): 23–30.CrossRef
  Zhou K, Zhang X, Zhang F L, Li Z Y. 2011. Phylogenetically diverse cultivable fungal community and polyketide synthase (PKS), non-ribosomal peptide synthase (NRPS) genes associated with the South China Sea sponges. Microb. Ecol., 62 (3): 644–654.CrossRef
  
• 作者单位：Yi Zhang 张翼 (1) (2) (3) (4)
  Jinyuan Han 韩金媛 (2)
  Yan Feng 冯妍 (1)
  Jun Mu 穆军 (5)
  Haiyan Bao 鲍海燕 (2)
  Andreas Kulik (6)
  Stephanie Grond (4)
  
  1. College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang, 524088, China
  2. School of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian, 116028, China
  3. School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China
  4. Institute of Organic Chemistry, University of Tübingen, Tübingen, 72076, Germany
  5. Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, 316022, China
  6. Inter - Faculty Institute for Microbiology and Infectious Medicine, University of Tübingen, Tübingen, 72076, Germany
  
• 刊物主题：Oceanography;
• 出版者：Springer Berlin Heidelberg
• ISSN：1993-5005

文摘

Until recently, little was known about the fungi found in shark gills and their biomedicinal potential. In this article, we described the isolation, bioactivity, diversity, and secondary metabolites of bioactive fungi from the gill of a shark (Carcharodon carcharias). A total of 115 isolates were obtained and grown in 12 culture media. Fifty-eight of these isolates demonstrated significant activity in four antimicrobial, pesticidal, and cytotoxic bioassay models. Four randomly selected bioactive isolates inhibited human cancer cell proliferation during re-screening. These active isolates were segregated into 6 genera using the internal transcribed spacer-large subunit (ITS-LSU) rDNA-sequence BLAST comparison. Four genera, Penicillium, Aspergillus, Mucor, and Chaetomium were the dominant taxa. A phylogenic tree illustrated their intergenera and intragenera genetic diversity. HPLC-DAD-HRMS analysis and subsequent database searching revealed that nine representative strains produced diverse bioactive compound profiles. These results detail the broad range of bioactive fungi found in a shark’s gills, revealing their biopharmaceutical potential. To the best of our knowledge, this is the first study characterizing shark gill fungi and their bioactivity.