参考文献:1. Wu Y, Zhou H, Fan X et al (2015) Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells. Cell Res 25:67鈥?9 CrossRef 2. Cong L, Ran FA, Cox D et al (2013) Multiplex genome engineering using CRISPR/Cas systems. Science 339:819鈥?23 CrossRef 3. Mali P, Esvelt KM, Church GM (2013) Cas9 as a versatile tool for engineering biology. Nat Methods 10:957鈥?63 CrossRef 4. Jinek M, Chylinski K, Fonfara I et al (2012) A programmable dual-RNA鈥揼uided DNA endonuclease in adaptive bacterial immunity. Science 337:816鈥?21 CrossRef 5. Wang H, Yang H, Shivalila CS et al (2013) One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell 153:910鈥?18 CrossRef 6. Li D, Qiu Z, Shao Y et al (2013) Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat Biotechnol 31:681鈥?83 CrossRef 7. Long C, McAnally J, Shelton J et al (2014) Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA. Science 345:1184鈥?188 CrossRef 8. Wu Y, Liang D, Wang Y et al (2013) Correction of a genetic disease in mouse via use of CRISPR-Cas9. Cell Stem Cell 13:659鈥?62 CrossRef 9. Oatley JM, Brinster RL (2012) The germline stem cell niche unit in mammalian testes. Physiol Rev 92:577鈥?95 CrossRef 10. Kubota H, Brinster RL (2006) Technology insight: in vitro culture of spermatogonial stem cells and their potential therapeutic uses. Nat Clin Pract Endocrinol Metab 2:99鈥?08 CrossRef