Biological properties of a duck enteritis virus attenuated via serial passaging in chick embryo fibroblasts

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• 作者：Chenghuai Yang (1)
  Junping Li (1)
  Qihong Li (1)
  Ling Li (1)
  Miao Sun (1)
  Huijiao Li (1)
  Yecai Xia (1)
  Hanchun Yang (2)
  Kangzhen Yu (3)
  
  1. China Institute of Veterinary Drug Control ; No. 8 Zhongguancun South Street ; Haidian District ; Beijing ; 100081 ; People鈥檚 Republic of China
  2. Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture ; College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology ; China Agricultural University ; No. 2 Yuanmingyuan West Road ; Haidian District ; Beijing ; 100193 ; People鈥檚 Republic of China
  3. Ministry of Agriculture ; No. 11 Nongzhanguan Nanli ; Chaoyang District ; Beijing ; 100125 ; People鈥檚 Republic of China
  
• 刊名：Archives of Virology
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：160
• 期：1
• 页码：267-274
• 全文大小：1,883 KB
• 参考文献：1. Baudet AERF (1923) Mortality in ducks in the Netherlands caused by a filterable virus Fowl plague. Tijdschr Diergeneeskd 50:455鈥?59
  2. Damiani AM, Matsumura T, Yokoyama N, Mikami T, Takahashi E (2000) A deletion in the gI and gE genes of equine herpesvirus type 4 reduces viral virulence in the natural host and affects virus transmission during cell-to-cell spread. Virus Res 67:189鈥?02 CrossRef
  3. Dardiri AH (1969) Attenuation of duck plague virus and its propagation in cell culture. Arch Gesamte Virusforsch 27:55鈥?4 314" target="_blank" title="It opens in new window">CrossRef
  4. Dingwell KS, Brunetti CR, Hendricks RL, Tang Q, Tang M, Rainbow AJ, Johnson DC (1994) Herpes simplex virus glycoproteins E and I facilitate cell-to-cell spread in vivo and across junctions of cultured cells. J Virol 68:834鈥?45
  5. Flowers CC, O鈥機allaghan DJ (1992) The equine herpesvirus type 1 (EHV-1) homolog of herpes simplex virus type 1 US9 and the nature of a major deletion within the unique short segment of the EHV-1 KyA strain genome. Virology 190:307鈥?15 CrossRef
  6. Huang YX (1959) Study on duck plague-like disease. J South China Agric Univ 1:1鈥?2
  7. Jacobs L, Rziha HJ, Kimman TG, Gielkens AL, Van Oirschot JT (1993) Deleting valine-125 and cysteine-126 in glycoprotein gI of pseudorabies virus strain NIA-3 decreases plaque size and reduces virulence in mice. Arch Virol 131:251鈥?64 378630" target="_blank" title="It opens in new window">CrossRef
  8. Kaashoek MJ, Moerman A, Madic J, Rijsewijk FA, Quak J, Gielkens AL, van Oirschot JT (1994) A conventionally attenuated glycoprotein E-negative strain of bovine herpesvirus type 1 is an efficacious and safe vaccine. Vaccine 12:439鈥?44 CrossRef
  9. Kalaimathi R, Janakiram D (1989) Adaptation of a virulent strain of duck plague virus to chicken embryo fibroblast cell culture. Indian Vet J 66:1001鈥?004
  10. Kruger JM, Sussman MD, Maes RK (1996) Glycoproteins gl and gE of feline herpesvirus-1 are virulence genes: safety and efficacy of a gl-gE deletion mutant in the natural host. Virology 220:299鈥?08 318" target="_blank" title="It opens in new window">CrossRef
  11. Li Y, Huang B, Ma X, Wu J, Li F, Ai W, Song M, Yang H (2009) Molecular characterization of the genome of duck enteritis virus. Virology 391:151鈥?61 CrossRef
  12. Liu J, Chen P, Jiang Y, Wu L, Zeng X, Tian G, Ge J, Kawaoka Y, Bu Z, Chen H (2011) A duck enteritis virus-vectored bivalent live vaccine provides fast and complete protection against H5N1 avian influenza virus infection in ducks. J Virol 85:10989鈥?0998 CrossRef
  13. Lomniczi B, Watanabe S, Ben-Porat T, Kaplan AS (1984) Genetic basis of the neurovirulence of pseudorabies virus. J Virol 52:198鈥?05
  14. Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen Z, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Irzyk GP, Jando SC, Alenquer ML, Jarvie TP, Jirage KB, Kim JB, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, Lohman KL, Lu H, Makhijani VB, McDade KE, McKenna MP, Myers EW, Nickerson E, Nobile JR, Plant R, Puc BP, Ronan MT, Roth GT, Sarkis GJ, Simons JF, Simpson JW, Srinivasan M, Tartaro KR, Tomasz A, Vogt KA, Volkmer GA, Wang SH, Wang Y, Weiner MP, Yu P, Begley RF, Rothberg JM (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437:376鈥?80
  15. Matsumura T, O鈥機allaghan DJ, Kondo T, Kamada M (1996) Lack of virulence of the murine fibroblast adapted strain, Kentucky A (KyA), of equine herpesvirus type 1 (EHV-1) in young horses. Vet Microbiol 48:353鈥?65 378-1135(09)59999-3" target="_blank" title="It opens in new window">CrossRef
  16. Matsumura T, Kondo T, Sugita S, Damiani AM, O鈥機allaghan DJ, Imagawa H (1998) An equine herpesvirus type 1 recombinant with a deletion in the gE and gI genes is avirulent in young horses. Virology 242:68鈥?9 CrossRef
  17. Mettenleiter TC, Lukacs N, Rziha HJ (1985) Pseudorabies virus avirulent strains fail to express a major glycoprotein. J Virol 56:307鈥?11
  18. Mettenleiter TC, Zsak L, Kaplan AS, Ben-Porat T, Lomniczi B (1987) Role of a structural glycoprotein of pseudorabies in virus virulence. J Virol 61:4030鈥?032
  19. Mijnes JDF, de Groot RJ (1999) Structure-function analysis of the feline herpesvirus virulence factors gE and gI. Vet Microbiol 69:89鈥?1 378-1135(99)00093-0" target="_blank" title="It opens in new window">CrossRef
  20. Neidhardt H, Schroder CH, Kaerner HC (1987) Herpes simplex virus type 1 glycoprotein E is not indispensable for viral infectivity. J Virol 61:600鈥?03
  21. Otsuka H, Xuan X (1996) Construction of bovine herpesvirus-1 (BHV-1) recombinants which express pseudorabies virus (PRV) glycoproteins gB, gC, gD, and gE. Arch Virol 141:57鈥?1 CrossRef
  22. Petrovskis EA, Timmins JG, Gierman TM, Post LE (1986) Deletions in vaccine strains of pseudorabies virus and their effect on synthesis of glycoprotein gp63. J Virol 60:1166鈥?169
  23. Sandhu TS, Metwally SA (2008) Duck Virus Enteritis (Duck Plague). In: Saif YM (ed) Diseases of poultry. Blackwell Publishing, Singapore, pp 384鈥?93
  24. Sinzger C, Knapp J, Schmidt K, Kahl M, Jahn G (1999) A simple and rapid method for preparation of viral DNA from cell associated cytomegalovirus. J Virol Methods 81:115鈥?22 34(99)00058-0" target="_blank" title="It opens in new window">CrossRef
  25. Spieker JO, Yuill TM, Burgess EC (1996) Virulence of six strains of duck plague virus in eight waterfowl species. J Wildl Dis 32:453鈥?60 3558-32.3.453" target="_blank" title="It opens in new window">CrossRef
  26. Sussman MD, Maes RK, Kruger JM, Spatz SJ, Venta PJ (1995) A feline herpesvirus-1 recombinant with a deletion in the genes for glycoproteins gI and gE is effective as a vaccine for feline rhinotracheitis. Virology 214:12鈥?0 CrossRef
  27. van Engelenburg FA, Kaashoek MJ, Rijsewijk FA, van den Burg L, Moerman A, Gielkens AL, van Oirschot JT (1994) A glycoprotein E deletion mutant of bovine herpesvirus 1 is avirulent in calves. J Gen Virol 75(Pt 9):2311鈥?318 317-75-9-2311" target="_blank" title="It opens in new window">CrossRef
  28. Wang J, Hoper D, Beer M, Osterrieder N (2011) Complete genome sequence of virulent duck enteritis virus (DEV) strain 2085 and comparison with genome sequences of virulent and attenuated DEV strains. Virus Res 160:316鈥?25 CrossRef
  29. Wu Y, Cheng A, Wang M, Yang Q, Zhu D, Jia R, Chen S, Zhou Y, Wang X, Chen X (2012) Complete genomic sequence of Chinese virulent duck enteritis virus. J Virol 86:5965 CrossRef
  30. Wu Y, Cheng A, Wang M, Zhu D, Jia R, Chen S, Zhou Y, Chen X (2012) Comparative genomic analysis of duck enteritis virus strains. J Virol 86:13841鈥?3842 CrossRef
  31. Yang C, Li Q, Li J, Zhang G, Li H, Xia Y, Yang H, Yu K (2014) Comparative genomic sequence analysis between a standard challenge strain and a vaccine strain of duck enteritis virus in China. Virus Genes 48:296鈥?03 3-1009-9" target="_blank" title="It opens in new window">CrossRef
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798

文摘

To gain a better understanding of the genetic changes required for attenuation of duck enteritis virus (DEV), the Chinese standard challenge strain of DEV (DEV CSC) was serially passaged 80 times in chick embryo fibroblasts. We plaque-purified the virus after the 25th passage (DEV p25) and the 80th passage (DEV p80) and investigated its in vitro and in vivo properties. Average plaque sizes for DEV p25 and p80 were significantly smaller than those for their parental DEV CSC. The results from an in vivo experiment revealed that DEV p25 and p80 were avirulent in ducks and protected them from virulent DEV challenge. The complete genome sequence of DEV p80 was determined and compared with that of the parent virus. An 1801-bp deletion was identified in the genome of DEV p80, which affected the genes encoding gI and gE. Moreover, there were 11 base substitutions, which led to seven amino acid changes in open reading frames LORF9, UL51, UL9, UL7, UL4, ICP4 and US3. Further DNA sequence analysis showed that the 1801-bp deletion was also present in DEV p25. Our findings suggest that DEV gE and/or gI are nonessential for virus growth and might, as with other herpesviruses, play an important role in cell-to-cell spread and virulence. Our experiments provide more genetic information about DEV attenuation and further advance our understanding of the molecular basis of DEV pathogenesis.