Brain cholinesterase reactivation as a marker of exposure to anticholinesterase pesticides: a case study in a population of yellow-legged gull Larus michahellis (Naumann, 1840) along the northern coast of Portugal

详细信息    查看全文

• 作者：Cátia S. A. Santos ; Marta S. Monteiro…
• 关键词：Avian toxicity ; Biomarkers ; Carbamate pesticides ; Chemical reactivation ; Organophosphate pesticides ; Spontaneous reactivation
• 刊名：Environmental Science and Pollution Research
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：23
• 期：1
• 页码：266-272
• 全文大小：520 KB
• 参考文献：Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 7:248–254CrossRef
  Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88–95CrossRef
  Escartı́n E, Porte C (1996) Acetylcholinesterase inhibition in the crayfish Procambarus clarkii exposed to fenitrothion. Ecotoxicol Environ Saf 34:160–164CrossRef
  Fildes K, Astheimer LB, Story PBWA, Hooper MJ (2006) Cholinesterase response in native birds exposed to fenitrothion during locust control operation in eastern Australia. Environ Toxicol Chem 25:2964–2970CrossRef
  Fleischli MA, Franson JC, Thomas NJ, Finley DL, Riley WJ (2004) Avian mortality events in the United States caused by anticholinesterase pesticides: a retrospective summary of national wildlife health center records from 1980 to 2000. Arch Environ Contam Toxicol 46:542–550CrossRef
  Frasco MF, Fournier D, Carvalho F, Guilhermino L (2005) Do metals inhibit acetylcholinesterase (AChE)? Implementation of assay conditions for the use of AChE activity as a biomarker of metal toxicity. Biomarkers 10:360–375CrossRef
  Goldstein MI, Lacher TE, Woodbridge B, Bechard MJ, Canavelli SB, Zaccagnini ME, Cobb GP, Scollon EJ, Tribolet R, Hooper MJ (1999) Monocrotophos-induced mass mortality of Swainson’s hawks in Argentina, 1995–96. Ecotoxicology 8:201–214CrossRef
  Guilhermino L, Celeste Lopes M, Carvalho AP, Soares AM (1996) Inhibition of acetylcholinesterase activity as effect criterion in acute tests with juvenile Daphnia magna. Chemosphere 32:727–38CrossRef
  Guilhermino L, Barros P, Silva MC, Soares AMVM (1998) Should the use of inhibition of cholinesterases as a specific biomarker for organophosphate and carbamate pesticides be questioned? Biomarkers 3:157–163CrossRef
  Guitart R, Sachana M, Caloni F, Croubels S, Vandenbroucke V, Berny P (2010) Animal poisoning in Europe. Part 3: wildlife. The Veterinary Journal 183:260–265CrossRef
  Hill EF (2003) Wildlife toxicology of organophosphorus and carbamate pesticides. In: Hoffman DJ, Rattner BA, Burton GA, Cairns J (eds) Handbook of ecotoxicology. Lewis Publishers, Boca Raton, pp 281–312
  Hooper MJ, Detrich PJ, Weisskopf CP, Wilson BW (1989) Organophosphorus insecticide exposure in hawks inhabiting orchards during winter dormant-spraying. Bull Environ Contam Toxicol 42:651–659CrossRef
  Hunt KA, Hooper MJ, Littrell EE (1995) Carbofuran poisoning in herons: diagnosis using cholinesterase reactivation techniques. J Wildl Dis 31:186–192CrossRef
  Ludke JL, Hill EF, Dieter MP (1975) Cholinesterase (ChE) response and related mortality among birds fed ChE inhibitors. Arch Environ Contam Toxicol 3:1–21CrossRef
  Manahan SE (2003) Toxicological chemistry and biochemistry. Lewis Publishers, Boca Raton (FL)
  Martin AD, Norman G, Stanley PI, Westlake GE (1981) Use of reactivation techniques for the differential diagnosis of organophosphorus and carbamate pesticide poisoning in birds. Bull Environ Contam Toxicol 26:775–780CrossRef
  Maxwell DM, Brecht KM, Sweeney RE (2013) A common mechanism for resistance to oxime reactivation of acetylcholinesterase inhibited by organophosphorus compounds. Chem Biol Interact 203:72–76CrossRef
  Monserrat JM, Bianchini A (2000) Methodological and biological aspects to be considered in acetylcholinesterase reactivation assays using 2-PAM. Environ Toxicol Pharmacol 9:39–47CrossRef
  Oropesa AL, Perez-Lopez M, Hernandez D, Garcia JP, Fidalgo LE, Lopez-Beceiro A, Soler F (2007) Acetylcholinesterase activity in seabirds affected by the prestige oil spill on the Galician coast (NW Spain). Sci Total Environ 372:532–538CrossRef
  Osten JR, Soares AMVM, Guilhermino L (2005) Black-bellied whistling duck (dendrocygna autumnalis) brain cholinesterase characterization and diagnosis of anticholinesterase pesticide exposure in wild populations from mexico. Environ Contam Toxicol 24:313–317CrossRef
  Radic Z, Taylor P (2006) Structure and function of cholinesterases. In: Gupta RC (ed) Toxicology of organophosphate and carbamate pesticides. Elsevier Academic Press, Waltham, pp 161–186CrossRef
  Smith MR, Thomas NJ, Hulse C (1995) Application of brain cholinesterase reactivation to differentiate between organophosphorus and carbamate pesticide exposure in wild birds. J Wildl Dis 31:263–267CrossRef
  Stansley W (1993) Field results using cholinesterase reactivation techniques to diagnose acute anticholinesterase poisoning in birds and fish archives. Environ ContamToxicol 25:315–321
  Thompson HM, Walker CH, Hardy AR (1991) Inhibition of avian esterases by organophosphorus insecticides - problems of reactivation and storage. Arch Environ Contam Toxicol 20:509–513CrossRef
  Thompson HM (1999) Esterases as markers of exposure to organophosphates and carbamates. Ecotoxicology 8:369–384CrossRef
  Trudeau S, Cartier GS (2000) Biochemical methods to determine cholinesterase activity in wildlife exposed to pesticides. National Wildlife Research Centre, Québec, Canada
  Voet D, Voet JG (1990) Biochemistry. Jonh Wiley, New York, XVII, 1223 pp
  
• 作者单位：Cátia S. A. Santos (1) (2)
  Marta S. Monteiro (1)
  Amadeu M. V. M. Soares (1) (3)
  Susana Loureiro (1)
  
  1. Department of Biology & CESAM, University of Aveiro, 3810-193, Aveiro, Portugal
  2. Department of Biology, Terrestrial Ecology Unit, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium
  3. Programa de Pós-Graduação em Produção Vegetal, Universidade Federal do Tocantins, Campus de Gurupi. Rua Badejós, Zona Rural, Cx. Postal 66-CEP: 77402-970, Gurupi-TO, Brasil
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Atmospheric Protection, Air Quality Control and Air Pollution
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Industrial Pollution Prevention
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1614-7499

文摘

Between late 2010 to early 2011, an increased mortality in gulls was observed along the northern coast of Portugal, with individuals exhibiting neurologic disorders consistent with an eventual anticholinesterase pesticide poisoning event. To clarify if this mortality was related to organophosphate (OP) and/or carbamate (CB) poisoning, chemical and spontaneous cholinesterase (ChE) reactivation was tested in the brain of the yellow-legged gull (Larus michahellis). Initial brain ChE activity in L. michahellis was 40.92 ± 5.23 U/mg of protein (average ± SE). Following chemical and spontaneous reactivation, ChE activity increased in average 70.38 ± 48.59 % and 131.95 ± 92.64 %, respectively. ChE reactivation was found to decrease at increasing concentrations of the oxime pyridine-2-aldoxime methochloride and dilution factor, underscoring the importance of first optimizing the assay conditions prior to its use on bird species. These results suggest that birds analysed could have been exposed to OP and CB pesticide compounds and that in most cases CB exposure appeared to be the main cause of birds poisoning. These results are an important contribution to environmental monitoring as it demonstrates the suitability of L. michaellis as sentinel species of OP and CB pesticides within an urban environment.