Synthesis, Characterization, In Vivo Imaging, Hemolysis, and Toxicity of Hydrophilic Ag₂S Near-Infrared Quantum Dots

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• 作者：Jaber Javidi ; Azadeh Haeri ; Farshad Hosseini Shirazi…
• 关键词：Silver sulfide ; Quantum dots ; In vivo imaging ; Protein corona ; MTT Assay
• 刊名：Journal of Cluster Science
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：28
• 期：1
• 页码：165-178
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Catalysis; Inorganic Chemistry; Physical Chemistry; Nanochemistry;
• 出版者：Springer US
• ISSN：1572-8862
• 卷排序：28

文摘

Ag₂S quantum dots (Qds) are a new type of near-infrared Qds and hold great potential for in vivo imaging. There is great demand for versatile, practical, and environmentally-friendly processes for formation of hydrophilic Ag₂S Qds. The present study prepared hydrophilic Ag₂S near-infrared Qds using a simple, easy, one-step method. The Qds were characterized by TEM, XRD, FT-IR, UV–Visible and PL spectroscopy and the effect of the reaction variables of time, temperature, precipitation agent feeding rate, and S:Ag molar ratio on the size and PL spectrum of the Ag₂S Qds was investigated. The Qds exhibited near-infrared photoluminescence and good photostability, which was evaluated under different conditions. Cytotoxicity tests were carried out on human A549 and Hep G2 cell lines at concentrations of 6.25–200 µg/ml. MTT assay revealed that the Qds showed no significant toxicity. The effect of plasma and corona proteins on the hemolytic activity of Ag₂S Qds in four concentrations was evaluated for rat and human red blood cells. In vivo imaging showed that the Ag₂S Qds penetrated the body of mice with excellent brightness. The hydrophilic Ag₂S Qds can be safely applied as near-infrared optical imaging probes for nanodiagnostics or in vivo imaging.