Characterization of Penicillium Species Isolated from Grape Berries by Their Internal Transcribed Spacer (ITS1) Sequences and by Gas Chromatography–Mass Spectrometry Analysis of Geosmin Produ

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• 作者：Stéphane La Guerche ; Carole Garcia ; Philippe Darriet ; Denis Dubourdieu and Jacques Labarère
• 刊名：Current Microbiology
• 出版年：2004
• 出版时间：June 2004
• 年：2004
• 卷：48
• 期：6
• 页码：405-411
• 全文大小：199 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  
• 出版者：Springer New York
• ISSN：1432-0991

文摘

Geosmin (trans-1,10-dimethyl-trans-9-decalol), an earthy-musty compound, has been identified in wines and in grape juice, in which its presence is highly detrimental to the aromatic quality. Geosmin has a biological origin, and the analysis of rotten grape microflora has been done on two grape varieties (Semillon, Cabernet Sauvignon) from six parcels of the Bordeaux region over 3 years (1999, 2000, 2001). Forty-three Penicillium-related species have been analyzed by gas chromatography–mass spectrometry (GC–MS) for their geosmin production. GC–MS analysis has demonstrated that the earthy odor was always correlated with the presence of geosmin. Phenotypic characterization of Penicillium spp. being ambiguous, a molecular characterization by rDNA internal transcribed spacer (ITS1) sequencing was performed for all strains. The results evidenced that all strains producing geosmin belonged to only one species, P. expansum, and that the other strains, not producing geosmin, belonged to three species: P. purpurogenum, P. thomii, and Talaromyces wortmanii.