High-Throughput DNA Microarray Detection of Pathogenic Bacteria in Shallow Well Groundwater in the Kathmandu Valley, Nepal
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- 作者：Daisuke Inoue ; Takuji Hinoura ; Noriko Suzuki ; Junqin Pang…
- 刊名：Current Microbiology
- 出版年：2015
- 出版时间：January 2015
- 年：2015
- 卷：70
- 期：1
- 页码：43-50
- 全文大小：748 KB
- 参考文献：1. Asian Development Bank (2010) Project Rationale: Need and Demand. In: 2010 Kathmandu Valley water supply and wastewater system improvement (Project feasibility study: Final report). Government of Nepal, Ministry of Physical Planning & Works, Kathmandu Upatyaka Khanepani Limited, pp. 21-2
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Takuji Hinoura (2)
Noriko Suzuki (1)
Junqin Pang (3)
Rabin Malla (4)
Sadhana Shrestha (5)
Saroj Kumar Chapagain (4)
Hiroaki Matsuzawa (6)
Takashi Nakamura (6)
Yasuhiro Tanaka (5)
Michihiko Ike (3)
Kei Nishida (6)
Kazunari Sei (1) (2)
1. Department of Health Science, School of Allied Health Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara-Minami, Kanagawa, 252-0373, Japan
2. Environment and Medical Sciences Course, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara-Minami, Kanagawa, 252-0373, Japan
3. Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan
4. Center of Research for Environment Energy and Water (CREEW), Baluwatar, GPO Box 25563, Kathmandu, Nepal
5. Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511, Japan
6. International Research Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511, Japan - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Microbiology
Biotechnology - 出版者：Springer New York
- ISSN：1432-0991
文摘
Because of heavy dependence on groundwater for drinking water and other domestic use, microbial contamination of groundwater is a serious problem in the Kathmandu Valley, Nepal. This study investigated comprehensively the occurrence of pathogenic bacteria in shallow well groundwater in the Kathmandu Valley by applying DNA microarray analysis targeting 941 pathogenic bacterial species/groups. Water quality measurements found significant coliform (fecal) contamination in 10 of the 11 investigated groundwater samples and significant nitrogen contamination in some samples. The results of DNA microarray analysis revealed the presence of 1-7 pathogen species/groups, including 1-7 biosafety level 2 ones, in 9 of the 11 groundwater samples. While the detected pathogens included several feces- and animal-related ones, those belonging to Legionella and Arthrobacter, which were considered not to be directly associated with feces, were detected prevalently. This study could provide a rough picture of overall pathogenic bacterial contamination in the Kathmandu Valley, and demonstrated the usefulness of DNA microarray analysis as a comprehensive screening tool of a wide variety of pathogenic bacteria.