Ferrocenylnaphthalene Diimide-Based Electrochemical Detection of Aberrant Methylation in hTERT Gene

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• 作者：Shinobu Sato (1) (2)
  Toshiro Saeki (1)
  Tomoki Tanaka (1)
  Yusuke Kanezaki (1)
  Mana Hayakawa (3)
  Kazuya Haraguchi (3)
  Masaaki Kodama (3) (5)
  Tatsuji Nishihara (4) (5)
  Kazuhiro Tominaga (3) (5)
  Shigeori Takenaka (1) (2)
  
• 关键词：Methylation ; Electrochemical detection ; Ferrocene ; Hybridization ; hTERT gene
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2014
• 出版时间：October 2014
• 年：2014
• 卷：174
• 期：3
• 页码：869-879
• 全文大小：506 KB
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• 作者单位：Shinobu Sato (1) (2)
  Toshiro Saeki (1)
  Tomoki Tanaka (1)
  Yusuke Kanezaki (1)
  Mana Hayakawa (3)
  Kazuya Haraguchi (3)
  Masaaki Kodama (3) (5)
  Tatsuji Nishihara (4) (5)
  Kazuhiro Tominaga (3) (5)
  Shigeori Takenaka (1) (2)
  
  1. Department of Applied Chemistry, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu, Fukuoka, 804-8550, Japan
  2. Research Center for Biomicrosensing Technology, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu, Fukuoka, 804-8550, Japan
  3. Department of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Science, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan
  5. Oral Bioresearch Center, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan
  4. Department of Health Promotion, Division of Infections and Molecular Biology, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan
  
• ISSN：1559-0291

文摘

Since aberrant methylation at CpG sites is linked to the silencing of tumor suppressor genes, DNA methylation analysis is important for cancer diagnosis. We developed ferrocenylnaphthalene diimide (FND), which has two ferrocenyl moieties at the substituent termini, as an electrochemical indicator for hybridized DNA duplexes. In this study, we attempted to detect aberrant methylation of human telomerase reverse transcriptase gene (hTERT), an efficient cancer marker, using FND-based hybridization coupled with electrochemical detection via a multi-electrode chip.