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• 作者：Chin-Shang Ho (1)
  Huei-Chuan Shih (2)
  Ho-Yih Liu (3)
  Shau-Ting Chiu (4)
  Mei-Hui Chen (5)
  Li-Ping Ju (6)
  Ya-Zhu Ko (3)
  Yu-Shen Shih (7)
  Chaur-Tzuhn Chen (8)
  Tsai-Wen Hsu (9)
  Yu-Chung Chiang (3)
  
  1. Graduate Institute of Bioresources ; National Pingtung University of Science and Technology ; Pingtung ; 91201 ; Taiwan
  2. Department of Nursing ; Meiho University ; Pingtung ; 912 ; Taiwan
  3. Department of Biological Sciences ; National Sun Yat-sen University ; Kaohsiung ; 804 ; Taiwan
  4. Department of Biology ; National Museum of Natural Science ; Taichung ; 404 ; Taiwan
  5. Conservation Division ; Forestry Bureau ; Council of Agriculture ; Executive Yuan ; Taipei ; 100 ; Taiwan
  6. Botanical Garden Division ; Taiwan Forestry Research Institute ; Taipei ; 100 ; Taiwan
  7. Department of Life Sciences ; National Chung Hsing University ; Taichung ; 40227 ; Taiwan
  8. Department of Forestry ; National Pingtung University of Science and Technology ; Pingtung ; 912 ; Taiwan
  9. Endemic Species Research Institute ; Nantou ; 552 ; Taiwan
  
• 关键词：Keteleeria ; Conservation ; Microsatellites ; Simple sequence repeat markers ; Pinaceae
• 刊名：BMC Research Notes
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：7
• 期：1
• 全文大小：192 KB
• 参考文献：1. Chiang, YC, Shih, HC, Chang, LW, Li, WR, Lin, HY, Ju, LP (2011) Isolation of 16 polymorphic microsatellite markers from an endangered and endemic species, Podocarpus nakaii (Podocarpaceae). Am J Bot 98: pp. e306-e309 CrossRef
  2. Ho, CS, Chen, CT, Ko, YZ, Ge, XJ, Hung, KH, Hsu, TW, Chiang, YC (2012) Isolation and characterization of 15 microsatellite loci in four endangered Amentotaxus species (Taxaceae). Am J Bot 99: pp. e157-e159 CrossRef
  3. Ju, LP, Shih, HC, Chiang, YC (2012) Microsatellite primers for the endangered beech tree, Fagus hayatae (Fagaceae). Am J Bot 99: pp. e453-e456 CrossRef
  4. Huang, S, Chiang, YC, Schaal, BA, Chou, CH, Chiang, TY (2001) Organelle DNA phylogeography of Cycas taitungensis, a relict species in Taiwan. Mol Ecol 10: pp. 2669-2681 CrossRef
  5. Huang, S, Hsieh, HT, Fang, K, Chiang, YC (2004) Patterns of genetic variation and demography of Cycas taitungensis in Taiwan. Bot Rev 70: pp. 86-92 CrossRef
  6. Ju, LP, Kuo, CC, Chao, YS, Cheng, YP, Gong, X, Chiang, YC (2011) Microsatellite primers in the native perennial cycad, Cycas taitungensis (Cycadaceae). Am J Bot 98: pp. e84-e86 CrossRef
  7. Fang, JY, Chung, JD, Chiang, YC, Chang, CT, Chen, CY, Hwang, SY (2013) Divergent selection and local adaptation in disjunct populations of an endangered conifer, Keteleeria davidiana var. formosana (Pinaceae). PLoS One 8: pp. e70162 CrossRef
  8. Frankham, R, Ballou, JD, Briscoe, DA (2002) Introduction to Conservation Genetics. . Cambridge University Press, Cambridge, UK CrossRef
  9. Schneider, D, Wink, M, Sporer, F, Lounibos, P (2002) Cycads: their evolution, toxins, herbivores and insect pollinators. Naturwissenschaften 89: pp. 281-294 CrossRef
  10. Frankham, R (2003) Genetics and conservation biology. C R Biol 326: pp. S22-S29 CrossRef
  11. Luscombe, D, Yang, Y (2013) Keteleeria davidiana var. formosana. IUCN 2013. IUCN Red List of Threatened Species. pp. 1
  12. Farjon, A (1989) A second revision of the genus Keteleeria Carri猫re (Taxonomic notes on Pinaceae II). Notes Roy Bot Gard Edinburgh 46: pp. 81-99
  13. Li, HL, Hsuan, K (1994) Pinaceae. Flora of Taiwan, 1, Volume 2. Taipei, Taiwan, pp. 568-569
  14. Chiang YC: / Research Project: Identification the Genetic Units for Conservation and Restoration of Keteleeria davidiana var. formosana. Taipei, Taiwan: Council of Agriculture; 2012. [ / Research report for 101 annual science and technology program of forestry bureau]
  15. Kanehira, R (1936) Pinaceae. Formosan trees: Indigenous to the island (revised). Department of Forestry, Government Research Institute, Taipei, Formosa, pp. 39-41
  16. Zane, L, Bargelloni, L, Patarnello, T (2002) Strategies for microsatellite isolation: A review. Mol Ecol 11: pp. 1-6 CrossRef
  17. Liao, PC, Gong, X, Shih, HC, Chiang, YC (2009) Isolation and characterization of eleven polymorphic microsatellite loci from an endemic species, Piper polysyphonum (Piperaceae). Conserv Genet 10: pp. 1911-1914 CrossRef
  18. Hsu, TW, Shih, HC, Kuo, CC, Chiang, TY, Chiang, YC (2013) Characterization of 42 microsatellite markers from poison ivy, Toxicodendron radicans (Anacardiaceae). Int J Mol Sci 14: pp. 20414-20426 CrossRef
  19. Benson, G (1999) Tandem repeats finder: A program to analyze DNA sequences. Nucleic Acids Res 27: pp. 573-580 CrossRef
  20. Kalendar, R, Lee, D, Schulman, AH (2011) Java web tools for PCR, in silico PCR, and oligonucleotide assembly and analysis. Genomics 98: pp. 137-144 CrossRef
  21. Chiang, YC, Shih, HC, Huang, MC, Ju, LP, Hung, KH (2012) Characterization of microsatellite loci from Litsea hypophaea (Lauraceae), a tree endemic to Taiwan. Am J Bot 99: pp. e251-e254 CrossRef
  22. Peakall, R, Smouse, PE (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28: pp. 2537-2539 CrossRef
  23. Excoffier, L, Lischer, HEL (2010) Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10: pp. 564-567 CrossRef
  24. Yu, JH, Chen, CM, Tang, ZH, Yuan, SS, Wang, CJ, Zu, YG (2012) Isolation and characterization of 13 novel polymorphic microsatellite markers for Pinus koraiensis (Pinaceae). Am J Bot 99: pp. e421-e424 CrossRef
  25. Hung, KH, Lin, CY, Huang, CC, Hwang, CC, Hsu, TW, Kuo, YL, Wang, WK, Hung, CY, Chiang, TY (2012) Isolation and characterization of microsatellite loci from Pinus massoniana (Pinaceae). Bot Stud 53: pp. 191-196
  26. Ge, XJ, Hsu, TW, Hung, KH, Lin, CJ, Huang, CC, Huang, CC, Chiang, YC, Chiang, TY (2012) Inferring mutiple refugia and phylogeographical patterns in Pinus massoniana based on nucleotide sequence variation and DNA fingerprinting. PLoS One 7: pp. e43717 CrossRef
  27. Mariette, S, Chagn茅, D, L茅zier, C, Pastuszka, P, Raffin, A, Plomion, C, Kremer, A (2001) Genetic diversity within and among Pinus pinaster populations: comparison between AFLP and microsatellite markers. Heredity 86: pp. 469-479 CrossRef
  
• 刊物主题：Biomedicine general; Medicine/Public Health, general; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1756-0500

文摘

Background Keteleeria davidiana var. formosana (Pinaceae), Taiwan cow-tail fir, is an endangered species listed on the IUCN Red List of Threatened Species and only two populations remain, both on the Taiwan Island. Sixteen polymorphic microsatellite loci were developed in an endangered and endemic gymnosperm species, Keteleeria davidiana var. formosana, and were tested in an additional 6 taxa, K. davidiana var. calcarea, K. davidiana var. chienpeii, K. evelyniana, K. fortunei, K. fortunei var. cyclolepis, and K. pubescens, to evaluate the genetic variation available for conservation management and to reconstruct the phylogeographic patterns of this ancient lineage. Findings Polymorphic primer sets were developed from K. davidiana var. formosana using the modified AFLP and magnetic bead enrichment method. The number of alleles ranged from 3 to 16, with the observed heterozygosity ranging from 0.28 to 1.00. All of the loci were found to be interspecifically amplifiable. Conclusions These polymorphic and transferable loci will be potentially useful for future studies that will focus on identifying distinct evolutionary units within species and establishing the phylogeographic patterns and the process of speciation among closely related species.