Flow-Injection Chemiluminescence as a Means of Studying the Metabolism of Unbound Chromium(III) in Rabbit Blood with On-Line Microdialysis Sampling

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• 作者：Jiaohua Luo ; Zhujun Zhang ; Dinglong Chen ; Weiqun Shu and Zhouping Wang
• 关键词：Key words ; Microdialysis ; chromium(III) ; metabolism ; chemiluminescence.
• 刊名：Microchimica Acta
• 出版年：2005
• 出版时间：July 2005
• 年：2005
• 卷：150
• 期：3-4
• 页码：311-316
• 全文大小：115 KB

文摘

In this paper, we present an in-vivo, on-line, real-time analytical system for monitoring the metabolism of free chromium(III) in rabbit blood. This system includes microdialysis sampling, ion-exchange on-line separation and chemiluminescence detection. The results show that none of the co-existing substances in the blood, including protein and other small molecules, interfere with the determination. CrCl₃·6H₂O was administrated orally (0.5 g), and the microdialysis probe was utilized to sample rabbit blood with a perfusion rate of 10 µL min−1. The dialytic efficiency of chromium(III) under the experimental conditions was 18.1 ± 5.1 % (n=3). The concentration-time curve of chromium(III) is in accordance with the one-compartmental open model, the T_1/2 is 16.62 min.