Biocompatibility and in vivo osteogenic capability of novel bone tissue engineering scaffold A-W-MGC/CS

详细信息    查看全文

• 作者：Chen Li (1)
  Guo-Xian Wang (2)
  Zheng Zhang (3)
  Dan-Ping Liu (3)
  
  1. Biobank ; the First Affiliated Hospital of Liaoning Medical University ; Jinzhou ; 121001 ; China
  2. Department of Pharmacology ; Liaoning Medical University ; Jinzhou ; 121000 ; China
  3. Department of Orthopaedic Surgery ; First Affiliated Hospital of Liaoning Medical University ; No. 2 Wuduan Renmin Street Guta District ; Jinzhou ; 121001 ; China
  
• 关键词：Bone tissue engineering ; A ; W ; MGC/CS ; Bone defect ; Repair
• 刊名：Journal of Orthopaedic Surgery and Research
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：9
• 期：1
• 全文大小：1,602 KB
• 参考文献：1. P茅rez-S谩nchez, MJ, Ram铆rez-Glindon, E, Lled贸-Gil, M, Calvo-Guirado, JL, P茅rez-S谩nchez, C (2010) Biomaterials for bone regeneration. Med Oral Patol Oral Cir Bucal 15: pp. e517-e522 CrossRef
  2. Neman, J, Hambrecht, A, Cadry, C, Goodarzi, A, Youssefzadeh, J, Chen, MY, Jandial, R (2012) Clinical efficacy of stem cell mediated osteogenesis and bioceramics for bone tissue engineering. Adv Exp Med Biol 760: pp. 174-187 CrossRef
  3. Porter, JR, Henson, A, Ryan, S, Popat, KC (2009) Biocompatibility and mesenchymal stem cell response to poly (epsilon-caprolactone) nanowire surfaces for orthopedic tissue engineering. Tissue Eng Part A 15: pp. 2547-2559 CrossRef
  4. Caralla, T, Joshi, P, Fleury, S, Luangphakdy, V, Shinohara, K, Pan, H, Boehm, C, Vasanji, A, Hefferan, TE, Walker, E, Yaszemski, M, Hascall, V, Zborowski, M, Muschler, GF (2013) In vivo transplantation of autogenous marrow-derived cells following rapid intraoperative magnetic separation based on hyaluronan to augment bone regeneration. Tissue Eng Part A 19: pp. 125-134 CrossRef
  5. Ciapetti, G, Granchi, D, Baldini, N (2012) The combined use of mesenchymal stromal cells and scaffolds for bone repair. Curr Pharm Des 18: pp. 1796-1820 CrossRef
  6. Meinel, L, Hofmann, S, Betz, O, Fajardo, R, Merkle, HP, Langer, R, Evans, CH, Vunjak-Novakovic, G, Kaplan, DL (2006) Osteogenesis by human mesenchymal stem cells cultured on silk biomaterials comparison of adenovirus mediated gene transfer and protein delivery of BMP-2. Biomaterials 27: pp. 4993-5002 CrossRef
  7. Xynos, ID, Hukkanen, MV, Batten, JJ, Buttery, LD, Hench, LL, Polak, JM (2000) Bioglass 45S5 stimulates osteoblast turnover and enhances bone formation in vitro: implications and applications for bone tissue engineering. Calcif Tissue Int 67: pp. 321-329 CrossRef
  8. Neman, J, Hambrecht, A, Cadry, C, Jandial, R (2012) Stem cell-mediated osteogenesis: therapeutic potential for bone tissue engineering. Biologics 6: pp. 47-57
  9. Da Li G, Da Zhou L, Pan TH, Chen GS, Lin Y, Mao M, Yan G: Effect of Mn-Zn ferrite on apatite-wollastonite glass-ceramic (A-W GC). / Biomed Mater 2009, 4:045001.
  10. Dunn, CA, Jin, Q, Taba, M, Franceschi, RT, Bruce Rutherford, R, Giannobile, WV (2005) BMP gene delivery for alveolar bone engineering at dental implants. Mol Ther 11: pp. 294-299 CrossRef
  11. Fujioka-Kobayashi, M, Ota, MS, Shimoda, A, Nakahama, K, Akiyoshi, K, Miyamoto, Y, Iseki, S (2012) Cholesteryl group- and acryloyl group-bearing pullulan nanogel to deliver BMP2 and FGF18 for bone tissue engineering. Biomaterials 33: pp. 7613-7620 CrossRef
  12. Pelled, G, Snedeker, JG, Ben-Arav, A, Rigozzi, S, Zilberman, Y, Kimelman-Bleich, N, Gazit, Z, M眉ller, R, Gazit, D (2012) Smad8/BMP2-engineered mesenchymal stem cells induce accelerated recovery of the biomechanical properties of the Achilles tendon. J Orthop Res 30: pp. 1932-1939 CrossRef
  13. Gassner, D, Durham, D, Pfannenstiel, SC, Brough, DE, Staecker, H (2012) Canalostomy as a surgical approach for cochlear gene therapy in the rat. Anat Rec (Hoboken) 295: pp. 1830-1836 CrossRef
  14. Menendez, MI, Clark, DJ, Carlton, M, Flanigan, DC, Jia, G, Sammet, S, Weisbrode, SE, Knopp, MV, Bertone, AL (2011) Direct delayed human adenoviral BMP-2 or BMP-6 gene therapy for bone and cartilage regeneration in a pony osteochondral model. Osteoarthritis Cartilage 19: pp. 1066-1075 CrossRef
  15. Misteli, T, Spector, DL (1997) Application of the green fluorescent protein in cell biology and biotechnology. Nat Biotechnol 15: pp. 961-964 CrossRef
  16. Ye, XY, Niu, XM, Tang, NW, Xu, YH, Li, ZM, Yu, YF, Lu, S, Chen, SW (2012) Adenovirus mediated knockdown of bone morphogenetic protein 2 inhibits human lung cancer growth and invasion in vitro and in vivo. Int J Immunopathol Pharmacol 25: pp. 967-976
  17. Liu, D, Hu, L, Zhang, Z, Li, QY, Wang, G (2013) Construction of human BMP2-IRES-HIF1伪mu adenovirus expression vector and its expression in mesenchymal stem cells. Mol Med Report 7: pp. 659-663
  18. Amini, AR, Laurencin, CT, Nukavarapu, SP (2012) Bone tissue engineering: recent advances and challenges. Crit Rev Biomed Eng 40: pp. 363-408 CrossRef
  19. Kohgo, T, Yamada, Y, Ito, K, Yajima, A, Yoshimi, R, Okabe, K, Baba, S, Ueda, M (2011) Bone regeneration with self-assembling peptide nanofiber scaffolds in tissue engineering for osseointegration of dental implants. Int J Periodontics Restorative Dent 31: pp. e9-e16
  20. Lee, SS, Huang, BJ, Kaltz, SR, Sur, S, Newcomb, CJ, Stock, SR, Shah, RN, Stupp, SI (2013) Bone regeneration with low dose BMP-2 amplified by biomimetic supramolecular nanofibers within collagen scaffolds. Biomaterials 34: pp. 452-459 CrossRef
  
• 刊物主题：Orthopedics; Surgical Orthopedics;
• 出版者：BioMed Central
• ISSN：1749-799X

文摘

Background This study aims to investigate the biocompatibility and in vivo osteogenic capability of the novel bone tissue engineering scaffold apatite-wollastonite-magnetic glass ceramic/chitosan (A-W-MGC/CS). Methods Rabbit bone marrow stromal cells (BMSCs) were transfected with adenovirus-human bone morphogenetic protein-2-green fluorescent protein (Ad-hBMP2-GFP). The transfected BMSCs were then inoculated onto the scaffold material A-W-MGC/CS to construct tissue-engineered bone. The attachment and proliferation of BMSCs were observed by scanning electron microscopy (SEM) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) detection, respectively. Rabbit models of bone defects were established and divided into three groups. Experimental group 1 was implanted with prepared tissue-engineered bone. Experimental group 2 was implanted with A-W-MGC/CS without transfected BMSCs. The blank group was injected with transfected BMSCs, without implantation of any scaffold. In the 12th week after surgery, the repair of bone defect was observed by X-ray examination, and histological observations of the area of bone defect were performed. Results A-W-MGC/CS resulted in good BMSC attachment and had no obvious effects on cell proliferation. In experimental group 1, good repair of bone defect was observed, and the scaffold material degraded completely. In experimental group 2, new bone was formed, but its quality was poor. In the blank group, there was mainly filling of fibrous connective tissues with no observable bone defect repair. Conclusion A-W-MGC/CS possesses good biocompatibility and in vivo osteogenic capability for bone defect repair.