Colorimetric determination of islet amyloid polypeptide fibrils and their inhibitors using resveratrol functionalized gold nanoparticles

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• 作者：Juan Zhang ; Yangyang Chen ; Defeng Li ; Ya Cao ; Zhaoxia Wang ; Genxi Li
• 关键词：Diabetes ; IAPP ; FTIR ; Dynamic light scattering ; Zeta potential ; Field emission electron microscopy ; Energy ; dispersive spectroscopy
• 刊名：Microchimica Acta
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：183
• 期：2
• 页码：659-665
• 全文大小：1,386 KB
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• 作者单位：Juan Zhang (1)
  Yangyang Chen (1)
  Defeng Li (1)
  Ya Cao (1)
  Zhaoxia Wang (2)
  Genxi Li (1) (3)
  
  1. Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai, 200444, People’s Republic of China
  2. Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210011, People’s Republic of China
  3. State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, Nanjing University, Nanjing, 210093, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

The article describes the preparation of gold nanoparticles functionalized with resveratrol (Res-AuNPs) in a single step and by integrating synthesis and modification. By using Res-AuNPs, we have established a colorimetric method for the determination of fibrillar islet amyloid polypeptides (IAPP) and its inhibitors. It is based on the specific recognition of resveratrol by IAPP fibrils. This results in a decrease in the concentration of Res-AuNPs in the supernatant and a corresponding reduction in absorbance at 537 nm. This finding was exploited to design a simple and sensitive colorimetric assay for IAPP fibrils. The analytical range extends from 30 to 400 μM, and the limit of detection is as low as 1.2 μM. In addition, the inhibitory effects of caffeic acid and caffein on the formation of fibrillar IAPP were evaluated. The method is simple and effective, and therefore is perceived to represent a promising scheme for determination of fibrillar IAPPs and their inhibitors.