Protein aggregation and membrane lipid modifications under lactic acid stress in wild type and OPI1 deleted Saccharomyces cerevisiae strains
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- 作者：Nadia Maria Berterame ; Danilo Porro ; Diletta Ami…
- 关键词：Saccharomyces cerevisiae ; Lactic acid ; Cell membrane ; FTIR ; Protein aggregation ; OPI1
- 刊名：Microbial Cell Factories
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：15
- 期：1
- 全文大小：1,410 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology
Applied Microbiology
Environmental Engineering/Biotechnology - 出版者：BioMed Central
- ISSN：1475-2859
文摘
Background Lactic acid is a versatile chemical platform with many different industrial applications. Yeasts have been demonstrated as attractive alternative to natural lactic acid producers since they can grow at low pH, allowing the direct purification of the product in the desired acidic form. However, when very high concentrations of organic acids are reached, the major limitation for a viable production is the toxic effect of the product. The accumulation in the cytosol of H+ and of the weak organic counter-anions triggers a cellular reprogramming. Here, the effects of lactic acid exposure on Saccharomyces cerevisiae have been evaluated by Fourier transform infrared (FTIR) microspectroscopy. In addition to -omic techniques, describing these responses in terms of systems and networks, FTIR microspectroscopy allows a rapid acquisition of the cellular biochemical fingerprint, providing information on the major classes of macromolecules.